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comparative gel filtration of toxin precursor and trypsin-activated toxin of clostridium botulinum type e.sakaguchi, genji (national institute of health, tokyo, japan), sumiko sakaguchi, and nobuko imai. comparative gel filtration of toxin grecursor and trypsin-activated toxin of clostridium botulinum type e. j. bacteriol. 87:401-407. 1964.-precursor of type e botulinus toxin was highly purified from bacterial cells by extraction, ammonium sulfate precipitation, ribonuclease digestion, and chromatography on cm-sephadex. the sample free from ribonucleic acid had a toxicity of 5.1 x 10(5)ld(50) per mg ...196414151063
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