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detection of mycobacterium avium subsp. paratuberculosis in a sheep flock in tuscany.paratuberculosis is an infectious disease which affects ruminants. in this work, a sheep flock in garfagnana district (tuscany, italy) was examined by agar gel immune-diffusion (agid) tests, culture, and pcr from feces, milk, tissue samples, and cheeses. at the first agid test, 7/280 (2.5 %) animals were positive. from these animals, feces and milk samples were collected: 4/7 feces (57.14 %) and 2/7 milk samples (28.57 %) were positive to culture and 7/7 (100 %) feces samples and 1/7 milk sample ...201526245917
detection of mycobacterium avium subsp. paratuberculosis in cheeses from small ruminants in tuscany.paratuberculosis is an infectious disease which affects mainly domestic and wild ruminants caused by mycobacterium avium subsp. paratuberculosis (map). map has been associated with human diseases like crohn disease, type-1 diabetes, sarcoidosis, multiple sclerosis and hashimoto's thyroiditis. the aim of this study was to determine the level of map positivity of cheeses produced in tuscany (italy) as an indication of human exposure to the specific pathogen. sampling was focused on artisanal chees ...201626555160
serological, culture and molecular survey of mycobacterium avium paratuberculosis in a goat flock in tuscany.mycobacterium avium paratuberculosis (map) is a pathogen which causes a chronic progressive granulomatous enteritis known as paratuberculosis or johne's disease and it primarily affects wild and domestic ruminants. the aim of this research was to examine a flock which consisted of 294 goats and was located in garfagnana district (tuscany, italy) performing elisa tests, culture and is900 pcr assay; direct diagnostic methods were carried out not only on bulk tank milk and cheese samples but also o ...201728353080
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