| quantitation of grapevine leafroll associated virus-1 and -3, grapevine virus a, grapevine fanleaf virus and grapevine fleck virus in field-collected vitis vinifera l. 'nebbiolo' by real-time reverse transcription-pcr. | taqman one-step real-time qrt-pcr assays were developed for the quantitation of grapevine leafroll associated virus-1 and -3 (glrav-1 and -3), grapevine virus a (gva), grapevine fanleaf virus (gflv) and grapevine fleck virus (gfkv) in vitis vinifera l. virus load in the progenies of three 'nebbiolo' clones planted in two experimental vineyards in piemonte (northwestern italy), and carrying the viruses in different combinations, was evaluated. quantitation primers were designed on the rna-depende ... | 2010 | 21167205 |