| viral isolates of a novel putative phlebovirus in the marche region of italy. | thirty pools from 900 (540 females and 360 males) phlebotomus perfiliewi sandflies collected during the summer of 2012 in the fermo area (marche region, central italy) were tested for the presence of phleboviruses. a nested polymerase chain reaction was performed using degenerated primers amplifying a fragment of the polymerase gene (large segment) and a fragment of the nucleoprotein gene (small segment) of the genus phlebovirus. one pool was positive for toscana virus, as expected from results ... | 2014 | 24534812 |