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canine filarial infections in liguria, north-west italy.this paper reports the findings of a study on the presence of various species of filarial nematodes in dogs in liguria, north-west italy, a region traditionally considered free from the disease. between 2009 and 2012 blood samples were taken from 365 dogs in rural areas in liguria. the blood samples were then submitted to knott's test, histochemical staining, polymerase chain reaction (pcr) and the enzyme-linked immunosorbent assay (elisa) for dirofilaria immitis antigens. overall, 35 of the 365 ...201625758206
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