molecular detection of brucella species in patients suspicious of brucellosis from zanjan, iran.brucella is an intracellular pathogen capable of infecting animals and humans. the aim of this study was to identify brucella spp in sera of high risk individuals by a polymerase chain reaction (pcr)-based method. a total of 180 patients suspected to have brucellosis were examined by serological tests. to establish a pcr protocol for diagnosis of active brucellosis, dna was extracted from the serum samples by using a commercial kit. pcr amplification was done for detection of brocella dna using ...201425242938
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