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mastitis diagnosis in dairy cows using pathoproof real-time polymerase chain reaction assay in comparison with conventional bacterial culture in a northern german field study.in the following field study, the commercial pathoproof mastitis pcr assay, a real-time pcr for identifying eleven mastitis pathogens and the staphylococcal beta-lactamase gene, was compared with conventional bacterial culture. for this purpose, 681 udder quarter samples from 173 clinically healthy cows with varying somatic cell count from four dairy herds in the region of osnabrück, lower saxony, germany, were collected between july 2010 and february 2011 and subjected to pcr and bacterial cult ...201223227767
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