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detection of tick blood parasites in egypt using pcr assay ii- borrelia burgdorferi sensu lato.the prevalence of borrelia burgdorferi sensu lato (s.l.), the etiologic agent of lyme borrelosis (lb), was determined for the first time in egypt by using polymerase chain reaction (pcr). questing 5243 hard and soft ticks were collected from animal farms throughout giza governorate. dna from 500 individual tick species was extracted and pcr was performed. primers verified from the sequence of german strain pko of borrelia afzelii were used. fragments of 642 bp were generated and sequenced. the p ...201021268526
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