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a duplex quantitative real-time pcr assay for the detection of haplosporidium and perkinsus species in shellfish.a duplex quantitative real-time polymerase chain reaction (dq-pcr) assay was optimized to simultaneously detect haplosporidium spp. and perkinsus spp. of shellfish in one reaction. two sets of specific oligonucleotide primers for haplosporidium spp. and perkinsus spp., along with two hydrolysis probes specific for each parasite group, were used in the assay. the dq-pcr results were detected and analyzed using the light cycler 2.0 software system. the dq-pcr identified and differentiated the two ...201323371501
temporal and spatial variation in the abundance of total and pathogenic vibrio parahaemolyticus in shellfish in china.we investigated the abundance of total and pathogenic vibrio parahaemolyticus in shellfish sampled from four provinces in china during may 2013 and march 2014 using the most probable number-polymerase chain reaction (mpn-pcr) method. total v. parahaemolyticus was detected in 67.7% of 496 samples. a total of 38.1% and 10.1% of samples exceeded 1,000 mpn g(-1) and 10,000 mpn g(-1), respectively. v. parahaemolyticus densities followed a seasonal and geographical trend, with guangxi and sichuan shel ...201526061712
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