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an indirect elisa for detection of theileria spp. antibodies using a recombinant protein (rtlsp) from theileria luwenshuni.theileria is a tick-borne, intracellular protozoan parasite of worldwide economic and veterinary importance in small ruminants. here, an enzyme-linked immunosorbent assay (elisa) was developed based on theileria luwenshuni recombinant surface protein (rtlsp) and was used in the standardization and validation of an elisa for the detection of circulating antibodies against ovine and caprine theileriosis. a total of 233 sera samples were used for the calculation of the cut-off value which served as ...201627048941
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