| molecular characterization of the hippeastrum chlorotic ringspot virus l segment and its protein. | hippeastrum chlorotic ringspot virus (hcrv) is a novel tospovirus that was identified in yunnan province, china, in 2013. we have sequenced the hcrv l gene, which is 8909 nt long and encodes the rna-dependent rna polymerase (2873 amino acids, 330.8 kda). the hcrv l protein shared highest similarity (89.4 %) with that of tomato yellow ring virus. the l protein contains a negative-sense rna virus rna-directed rna polymerase motif and an endonuclease domain at the n-terminus. combined with our prev ... | 2014 | 24841760 |
| a new tospovirus causing chlorotic ringspot on hippeastrum sp. in china. | a new tospovirus, hcrv 2007-zdh, was isolated from a hippeastrum sp. plant displaying necrotic and chlorotic ringspot symptoms in yunnan province. this virus isolate was characterized based on particle morphology and rna sequences analyses. quasi-spherical, enveloped particles measuring about 70-100 nm, typical of tospoviruses, were observed in sap and cells of the infected plants. transmission studies by inoculating this isolate mechanically to hippeastrum sp. confirmed that 2007-zdh is the cau ... | 2013 | 23306942 |