| development of a recombinant oppa-based indirect hemagglutination test for the detection of antibodies against haemophilus parasuis. | an indirect hemagglutination (iha) test that could detect antibodies against haemophilus parasuis was developed. the full-length cdna sequence of the oligopeptide permease abc transporter membrane protein (oppa) gene was cloned, and inserted into the prokaryotic expression vector pet-30a(+) to construct recombinant plasmid pet-30a-oppa. the recombinant oppa protein was expressed partly in soluble form in escherichia coli bl21 (de3) and then purified by ni(2+) column. furthermore, the recombinant ... | 2015 | 25910625 |