identification and genomic characterization of a novel fish reovirus, hubei grass carp disease reovirus, isolated in 2009 in china. | a novel fish reovirus, hubei grass carp disease reovirus (hgdrv; formerly grass carp reovirus strain 104, gcrv104), was isolated from diseased grass carp in china in 2009 and the full genome sequence was determined. this reovirus was propagated in a grass carp kidney cell line with a typical cytopathic effect. the total size of the genome was 23 706 bp with a 51 mol% g+c content, and the 11 dsrna segments encoded 12 proteins (two proteins encoded by segment 11). a nucleotide sequence similarity ... | 2013 | 23851441 |
complete genome sequence and comparative analysis of grass carp reovirus strain 109 (gcrev-109) with other grass carp reovirus strains reveals no significant correlation with regional distribution. | a new grass carp reovirus strain, tentatively named gcrev-109, was isolated in hubei, china, and its complete genome sequence was determined. the genome contained 11 double-stranded rna segments (s1-s11) covering 24,620 base pairs. all of the segments had conserved terminal nucleotides, with guaa(u)/cu at the 5' end and ucauc at the 3' end. protein sequence comparison showed that gcrev-109 was most closely related to gcrv-gd108 and shared 96.6-99.5 % protein sequence identity but only shared 16. ... | 2014 | 24687858 |