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bioassay-directed fractionation of pah of molecular mass 302 in coal tar-contaminated sediment.bioassay-directed fractionation was used to characterise genotoxic polycyclic aromatic hydrocarbons (pah) of molecular mass 302 amu in organic solvent extracts of coal tar-contaminated sediment from sydney harbour, nova scotia. a normal phase hplc technique was employed to separate pah-rich solvent extracts into fractions containing pah of single molecular mass classes. the 302 amu molecular mass fraction was isolated and further separated using reversed phase hplc; subfractions were collected e ...199910472130
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