first characterization of borrelia burgdorferi sensu lato from ticks and skin biopsy in bulgaria.lyme borreliosis is the most frequent tick-borne disease in the northern hemisphere. here we describe the first isolation of borrelia burgdorferi sensu lato in bulgaria: the midguts of 47 ixodes ricinus obtained by flagging from the central park in sofia, bulgaria were cultivated for borreliae in bsk medium. the eight isolates obtained from the ticks and one skin isolate from a bulgarian patient with erythema migrans were subjected to phenotypic [outer surface protein a (ospa) serotyping] and ge ...19989574899
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