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dengue virus type 3 in rio de janeiro, brazil.dengue virus type 3 was isolated for the first time in the country as an indigenous case from a 40 year-old woman presenting signs and symptoms of a classical dengue fever in the municipality of nova iguaçu, state of rio de janeiro. this serotype has been associated with dengue haemorrhagic epidemics and the information could be used to implement appropriate prevention and control measures. virological surveillance was essential in order to detected this new serotype.200111685256
dengue virus type 3 isolation from aedes aegypti in the municipality of nova iguaçu, state of rio de janeiro.in a prospective field study conducted from july 2000 to june 2001, adult aedes aegypti and ae. albopictus mosquitoes were caught from the municipality of nova iguaçu, state of rio de janeiro, brazil. virus isolation in ae. albopictus clone c6/36 cell line and a semi-nested reverse transcription-polymerase chain reaction detected only dengue virus type 3 in three pools of ae. aegypti, despite the co-circulation of den-1, den-2 and den-3 serotypes in that area. no viruses were detected in ae. alb ...200212386700
dengue virus type 3, brazil, 2002.during the summer of 2002, rio de janeiro had a large epidemic of dengue fever; 288,245 cases were reported. a subset of 1,831 dengue hemorrhagic fever cases occurred. in this study, performed in the first half of 2002, samples from 1,559 patients with suspected cases of dengue infection were analyzed. from this total, 1,497 were obtained from patients with nonfatal cases, and 62 were obtained from patients with fatal cases. by the use of different methods, 831 (53.3%) cases, including 40 fatal ...200516229765
complete genetic characterization of a brazilian dengue virus type 3 strain isolated from a fatal outcome.we have determined the complete nucleotide and the deduced amino acid sequences of brazilian dengue virus type 3 (denv-3) from a dengue case with fatal outcome, which occurred during an epidemic in the state of rio de janeiro, brazil, in 2002. this constitutes the first complete genetic characterization of a brazilian denv-3 strain since its introduction into the country in 2001. denv-3 was responsible for the most severe dengue epidemic in the state, based on the highest number of reported case ...200616862328
genetic characterization of dengue virus type 3 isolates in the state of rio de janeiro, 2001.the genetic characterization of dengue virus type 3 (den-3) strains isolated from autochthonous cases in the state of rio de janeiro, brazil, in 2001 is presented. restriction site-specific (rss)-pcr performed on 22 strains classified the brazilian den-3 viruses as subtype c, a subtype that contains viruses from sri lanka, india, africa and recent isolates from central america. nucleic acid sequencing (positions 278 to 2550) of one den-3 strain confirmed the origin of these strains, since genoty ...200212185377
quantification of dengue virus type 3 rna in fatal and non-fatal cases in brazil, 2002.we examined levels of dengue virus type 3 (denv-3) rna in association with the type of infection (primary or secondary) in 42 patients with fatal and non-fatal outcomes in rio de janeiro, brazil, 2002. subjects with fatal outcomes had mean virus titers significantly higher than those who survived (12.5 vs. 7.9 log(10) rna copies/ml). because primary infections were confirmed among the fatal cases (52.1%), antibody-dependent enhancement alone did not explain all the cases of severe disease in thi ...200919261315
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