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identification and quantification of the acanthamoeba species and genotypes from reservoirs in taiwan by molecular techniques.the occurrence of acanthamoeba was investigated from 21 main reservoirs of taiwan with 12 (57.1%) testing positive. analysis of the 18s rrna gene pcr product was performed in order to identify the acanthamoeba isolates. acanthamoeba spp. concentrations were determined according to taqman real-time qpcr. acanthamoeba genotypes of all isolates were identified t4. the species were categorized to acanthamoeba culbertsoni, acanthamoeba polyphaga, acanthamoeba castellanii and acanthamoeba hatchetti. t ...201424388954
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