| comparison of smartcycler real-time reverse transcription-pcr assay in a public health laboratory with direct immunofluorescence and cell culture assays in a medical center for detection of influenza a virus. | a single-tube real-time (fluorogenic) reverse transcription (rt)-pcr with the smartcycler instrument (smartcycler rt-pcr) for influenza a virus detection was evaluated with 238 respiratory specimens. direct immunofluorescence antibody staining (dfa) and primary rhesus monkey kidney cell culture were performed on-site at yale-new haven hospital. specimens were transported to the connecticut department of public health laboratory for real-time rt-pcr. cell culture detected influenza a virus in all ... | 2003 | 12904361 |