development of a species-diagnostic marker and its application for population genetics studies of the stingless bee trigona collina in thailand.a molecular maker for authenticating species origin of the stingless bee (trigona collina) was developed. initially, amplified fragment length polymorphism analysis was made of 11 stingless bee species using 64 primer combinations. a 316-bp band found only in t. collina was cloned and sequenced. a primer pair (cutc1-f/r) was designed and tested for species-specificity in 15 stingless bee species (239 nests). the expected 259-bp fragment was consistently amplified in all t. collina individuals (1 ...201020486087
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