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detection and molecular characterization of babesia, theileria, and hepatozoon species in hard ticks collected from kagoshima, the southern region in japan.to reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (pcr) system to detect the most common agents of tick-borne parasitic diseases, namely babesia, theileria, and hepatozoon parasites. we collected host-seeking or animal-feeding ticks in kagoshima prefecture, the southernmost region of kyusyu island in southwestern japan. twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6kbp) for ...201728501503
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