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characterization of an h5n8 influenza a virus isolated from chickens during an outbreak of severe avian influenza in japan in april 2014.a highly pathogenic avian influenza virus (hpaiv) of subtype h5n8, a/chicken/kumamoto/1-7/2014, was isolated from a japanese chicken farm during an outbreak in april 2014. phylogenetic analysis revealed that this virus belonged to ha clade 2.3.4.4. all eight genomic segments showed high sequence similarity to those of the h5n8 subtype hpaivs a/broiler duck/korea/buan2/2014 and a/baikal teal/korea/donglim3/2014, which were isolated in korea in january 2014. intranasal experimental infection of ch ...201525902725
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