a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family.a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ...200111402861
determination and risk assessment of sixteen polycyclic aromatic hydrocarbons in vegetables.polycyclic aromatic hydrocarbons (pahs) are a group of organic environmental pollutants posing a potential risk to human health. this study was constructed to investigate the presence of 16 pahs in six commonly consumed vegetables collected from the markets in shandong, china by a quick, easy, cheap, effective, rugged, safe (quechers)-based extraction method coupled with gas chromatography-mass spectrometry (gc-ms). our results showed that the vegetables were polluted with pahs at an alarming le ...201829087788
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