cryopreservation of third-stage larvae of brugia malayi and dipetalonema viteae. | methods are described for the cryopreservation of third-stage larvae of brugia malayi. optimum conditions utilized larvae free from the mosquito host frozen at the rate of -1 degree or -0.8 degrees c per min in medium containing 9% dimethyl sulfoxide and 0.004 m polyvinylpyrrolidone. nonfrozen or thawed larvae were inoculated intraperitoneally into jirds (meriones unguiculatus), the thawed larvae after cryogenic storage for 5-378 days. in general, the percentage of adult worms recovered at necro ... | 1983 | 6683940 |