| specificity and sensitivity of skin test reactions to extracts of toxocara canis and ascaris suum ii. homologous 48-hour passive cutaneous anaphylaxis tests with sera from infected guinea pigs. | the specificity and sensitivity of adult and larval somatic antigens and perienteric fluid of toxocara canis and ascaris suum were investigated by using a modified passive cutaneous anaphylaxis procedure in guinea pigs. pooled sera from animals infected with low doses (0.01, 0.1, or 1.0 egg/g) were most reactive with the homologous larval antigen preparation. however, the toxocara antisera were highly reactive with this antigen only, whereas the ascaris antisera reactions could not be interprete ... | 1975 | 50748 |
| immunopathology of toxocara canis and ascaris suum infections of the eye: the role of the eosinophil. | intravitreal infection of guinea pigs with second-stage larvae of toxocara canis and second- and fourth-stage larvae of ascaris suum induced intraocular ige antibodies and a dense eosinophil infiltration in the anterior chamber and throughout the uveal tract. the eosinophil infiltrate began within 1 day after infection and persisted for as long as 51 days. by day 12 after intravitreal infection, the injected ascarid larvae were surrounded by granulomas which consisted almost entirely of eosinoph ... | 1979 | 511459 |
| the comparison of counterimmunoelectrophoresis with indirect haemagglutination test for detection of antibodies in experimentally infected guinea pigs with toxocara canis. | the humoral response of guinea pigs infected with doses of 500, 1000, and 1500 infective toxocara canis ova were measured by counterimmunoelectrophoresis and indirect haemmagglutination tests one to five weeks after infection. the antigens used in these measurements were prepared from adult t. canis. counterimmunoelectrophoresis showed lower titres than haemagglutination method, but it was performed rapidly and with ease. the possible use of these techniques for immunodiagnosis of human visceral ... | 1977 | 886181 |
| ascaris suum and toxocara canis: egg extract antigens in guinea pigs and the macrophage migration inhibition test. | | 1976 | 987922 |
| specificity and sensitivity of skin test reactions to extracts of toxocara canis and ascaris suum. i. skin tests done on infected guinea pigs. | the specificity and sensitivity of adult and larval somatic antigens, and perienteric fluid of toxocara canis and ascaris suum were investigated by using intradermal skin tests in guinea pigs. these animals were infected with low doses (0.01, 0.1 or 1.0 egg/g) of these helminths. toxocara larval antigen (tl) induced larger reactions in toxocara-infected animals than did the other antigens, suggesting a superior sensitivity for this antigen. in addition, ascaris perienteric fluid (ap) provoked sk ... | 1975 | 1155689 |
| trichinella spiralis: a 76-kda excretory/secretory larval antigen identified by a monoclonal antibody. | spleen cells from balb/c mice were fused with myeloma cells following infection of the mice with trichinella spiralis larvae and an ip booster injection with larval homogenate antigen. a monoclonal antibody (mab), designated as ts 3g6 which did not react with sera or tissue extracts from noninfected mice, rats, and guinea pigs, was selected for further studies because of its high activity and specificity. when tested in elisa ts 3g6 did not cross-react with ascaris suum, a. lumbricoides, toxocar ... | 1991 | 1889470 |
| immediate hypersensitivity and serological responses in guinea pigs infected with toxocara canis or trichinella spiralis. | | 1965 | 4953925 |
| [localization in the brain of the guinea pig of 2nd stage larvae of toxocara canis (werner, 1782). electroencephalographic, parasitological and anatomo-histopathological documentation]. | | 1966 | 5991288 |
| in vitro interaction of eosinophils from ascarid-infected eyes with ascaris suum and toxocara canis larvae. | the role of eosinophils in ocular ascarid infection is studied in an animal model. primary intravitreal infection of guinea-pig eyes with ascarid (toxocara canis, ascaris suum) second-stage (l2) larvae resulted in an anterior chamber exudate containing 98% or more eosinophils. anterior chamber aspirates were cultured in rpmi media 1640 with t. canis or a. suum l2 larvae at 37 degrees c for 1-96 hours. the in vitro interaction of eosinophils with l2 larvae was studied by light, and scanning and t ... | 1983 | 6618795 |
| [serological demonstration of experimental round worm infections-ascaris suum, toxocara canis--in swine by means of the indirect immunofluorescence antibody test]. | by means of indirect immunofluorescent antibody reaction (ifar), using serum of experimentally infected pigs, various antigens were studied with regard to their usefulness for serological verification of prepatent ascaris suum and toxocara canis infections. eggs, egg larvae, larvae received from livers, lungs and brains of experimentally infected white mice and sections of adult t. canis as well as eggs, egg larvae, liver larvae and sections of frozen adult a. suum proved to be not suitable for ... | 1981 | 7039425 |
| [the larva precipitation test with ascaris suum and toxocara canis larvae]. | investigations were made by means of a larvae precipitation test (lpt) on different laboratory animals (pigs and white mice, and pigs, rabbits, guinea pigs and white mice respectively) experimentally infected with ascaris suum and toxocara canis respectively for the serological evidence of specific a. suum and t. canis antibodies. living a. suum larvae isolated from the lungs of experimentally infected white mice forming oral, excretion porus and cuticula precipitates proved to be a suitable ant ... | 1982 | 7081762 |
| immunopathology of ascarid infection of the eye. role of ige antibodies and mast cells. | the roles of ige antiascarid antibodies and mast cells were compared in passively sensitized guinea pigs and animals infected intravitreally with ascarid larvae (toxocara canis, ascaris suum). intravenous ige antibody disappeared from the serum within 48 hours, but induced a hypersensitive state that persisted for 28 days. in systemically immunized animals, the aqueous-serum ige antibody ratio was 1:1,000 or less. passive periocular anaphylactic reactions produced an infiltration of neutrophils ... | 1981 | 7295137 |
| toxocara canis-induced airway eosinophilia and tracheal hyporeactivity in guinea pigs and mice. | guinea pigs and mice infected with the parasitic worms toxocara canis developed airway inflammation and tracheal hyporesponsiveness. preceding inflammatory cell infiltration a brief hyperreactive response occurred in guinea pigs to histaminergic receptor stimulation at 3 days post infection (p.i.) and in mice to acetylcholine receptor stimulation at 1 day p.i. few but large eosinophilic inflammatory foci developed in guinea pigs at 14 days p.i. mice demonstrated progressive multifocal inflammati ... | 1995 | 8666037 |
| cytokines in the modulation of eosinophilia. | in this review we discuss our recently results showing interleukin 5 (il-5) involvement in eosinophil migration and in the maintenance of eosinophilia in blood, bone marrow, lung and peritoneal cavity, in a visceral larva migrans syndrome model using guinea-pigs infected with toxocara canis. we also describe the sequential release of tnf-alpha and il-8 during the course of infection. finally we propose a biological role for il-5, at least in our model, as a modulator of il-8 release and secretio ... | 1997 | 9698922 |
| interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation. | serum and balf (bronchoalveolar lavage fluid) il-8 levels and serum levels were investigated in toxocara canis infected guinea-pigs and the role of il-5 as a modulator of cytokine secretion was studied. serum levels increased early in infected animals, exceeding control levels 4 h after infection, peaked between days 6 and 18, and continued to exceed control levels after 48 days of infection. serum and balf il-8 levels showed the same profile as blood eosinophilia, increasing 6 days post-infecti ... | 1998 | 9839698 |
| new animal model for human ocular toxocariasis: ophthalmoscopic observation. | although human ocular toxocariasis causes severe vision defect, little is known about its aetiology, diagnosis, and treatment. to develop a new animal model for human ocular toxocariasis, ophthalmological findings of fundi in mongolian gerbils, meriones unguiculatus, and balb/c mice were investigated following infection with toxocara canis. | 1999 | 10413704 |
| disodium cromoglycate prevents ileum hyperreactivity to histamine in toxocara canis-infected guinea pigs. | the aim of this study was to investigate whether toxocara canis infection in guinea pigs provokes changes in ileum responsiveness to histamine. ileum segments from control and t. canis-infected groups were placed at isometric conditions and submitted to various doses of histamine. no changes were observed between controls and t. canis-infected groups at days 3, 6 and 12 after infection. however, at days 18 and 24 after infection, there was a significant increase in ileum responsiveness to histam ... | 2003 | 12967589 |
| amebic invasion of the liver of guinea pigs infected with the larvae of a nematode, toxocara canis. | | 1956 | 13365620 |
| nematode induced hypersenistivity reactions in guinea pigs: onset of eosinophilia and positive schultz-dale reactions following graded infections with toxocara canis. | | 1963 | 14088709 |
| il-5 drives eosinophils from bone marrow to blood and tissues in a guinea-pig model of visceral larva migrans syndrome. | this study was undertaken to evaluate the role of il-5 in eosinophil migration and in the maintenance of eosinophilia in a guinea-pig model of visceral larva migrans syndrome. the results show that the infection of animals with toxocara canis induced an early increase in serum il-5 levels that might be essential for eosinophil differentiation and proliferation and for the development of eosinophilia. when infected guinea-pigs were treated with mab anti-il-5 (trfk-5) given at the same time or 1 o ... | 1996 | 18475693 |
| development of some larval nematodes in experimental and natural animal hosts: an insight into development of pathological lesions vis-a-vis host-parasite interactions. | infective third-stage larvae of three spiruroid nematodes, ascarops strongylina and physocephalus sexalatus of pigs and spirocerca lupi of dogs, were recovered from 14 species of coprophagous beetles belonging to 4 different genera. these larvae were fed to rabbits and/or guinea pigs to study their development in these experimental hosts. larvae of a. strongylina reached the adult stage in all rabbits and one guinea pig. the adult worms recovered in these hosts were 40% and 4%, respectively, and ... | 2013 | 24453801 |