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rapid and sensitive detection of campylobacter spp. in chicken products by using the polymerase chain reaction.the polymerase chain reaction (pcr) after a short enrichment culture was used to detect campylobacter spp. in chicken products. after the 16s rrna gene sequence of campylobacter jejuni was determined and compared with known sequences from other enterobacteria, a primer and probe combination was selected from the region before v3 and the variable regions v3 and v5. with this primer set and probe, 426-bp fragments from c. jejuni, campylobacter coli, and campylobacter lari could be amplified. the d ...19921282312
chicken as potential contamination source of campylobacter lari in iquitos, peru.in order to know the importance of chicken as natural reservoir of campylobacter lari in iquitos, peru; samples were obtained by cloacal swabs from 200 chickens and immediately placed into a semisolid enrichment medium; these were streaked on modified skirrow agar. the organism was isolated from 21 (10.5%) samples, corresponding 58.8% to biovar i and 41.2% to biovar ii (lior scheme). the results provide evidence that chicken appear to be prominent reservoirs of campylobacter lari in iquitos.19947569622
ciprofloxacin resistant campylobacter spp. in humans: an epidemiological and laboratory study.from the end of april 1991 until the end of 1991, 2209 isolates of campylobacter spp. have been collected in plymouth phl of which 91 (4.1%) were resistant to ciprofloxacin. none of the 91 patients involved had taken a quinolone, but 30/91 (33%) had travelled abroad (16 to the iberian peninsula) in the three months preceding isolation of the ciprofloxacin-resistant campylobacter spp. in the case-control study 12/15 (80%) of the cases had recently consumed poultry as had 20/24 (83%) of controls w ...19968722540
specific detection and confirmation of campylobacter jejuni by dna hybridization and pcr.conventional detection and confirmation methods for campylobacter jejuni are lengthy and tedious. a rapid hybridization protocol in which a 1,475-bp chromogen-labelled dna probe (pdt1720) and campylobacter strains filtered and grown on 0.22-micron-pore-size hydrophobic grid membrane filters (hgmfs) are used was developed. among the environmental and clinical isolates of c. jejuni, campylobacter coli, campylobacter jejuni subsp. doylei, campylobacter lari, and arcobacter nitrofigilis and a panel ...19979361442
differentiation of campylobacter coli and c. jejuni by length and dna sequence of the 16s-23s rrna internal spacer region.the internal spacer region (isr) between the 16s and 23s rrna genes of campylobacter was investigated by pcr fragment length typing and dna sequencing of clinical and chicken wild-type isolates. pcr fragment length typing showed one fragment of 859 nt in length for the 12 strains of campylobacter coli investigated. thirty-six of the campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. three strains showed two fragments between 501 and 92 ...199910206715
detection of thermophilic campylobacter from sparrows by multiplex pcr: the role of sparrows as a source of contamination of broilers with campylobacter.the best combination of primers and the annealing temperature of multiplex pcr for campylobacter jejuni, campylobacter coli, and campylobacter lari were examined. the multiplex pcr was able to detect type strains of the three species. all results of identification of wild strains (30 strains of c. jejuni, 20 strains of c. coli, and 4 strains of c. lari) by the multiplex pcr coincided with those of the conventional biochemical identification tests, suggesting that the multiplex pcr can simultaneo ...200011193345
prevalence of thermophilic campylobacter spp. in ready-to-eat foods and raw poultry in northern ireland.although there have been numerous studies investigating the prevalence of campylobacters in animals and raw meats, there are limited data on the persistence of these organisms in ready-to-eat (rte) foodstuffs. although poultry is now well established as a major reservoir of thermophilic campylobacters, it is widely assumed that hazard analysis critical control point (haccp) controls in commercial and industrial settings are effective in eliminating this hazard through thorough cooking of rte pro ...200212182488
effect of incubation temperature on isolation of campylobacter jejuni genotypes from foodstuffs enriched in preston broth.preston broth and agar incubated at either 37 or 42 degrees c have been widely used to isolate campylobacters from foodstuffs. the consequences of using either incubation temperature were investigated. retail packs of raw chicken (n = 24) and raw lamb liver (n = 30) were purchased. samples were incubated in preston broth at 37 and 42 degrees c and then streaked onto preston agar and incubated as before. two campylobacter isolates per treatment were characterized. poultry isolates were genotyped ...200312902254
rapid detection of campylobacter jejuni in chicken rinse water by melting-peak analysis of amplicons in real-time polymerase chain reaction.five dna extraction protocols for the detection of campylobacter spp. by polymerase chain reaction (pcr) were compared. a method involving triton x-100 produced template dna of sufficient quality to allow the detection of campylobacter jejuni at levels of 100 cfu/ml in pure culture. primers were designed on the basis of the cadf gene sequence. with a sybr green i real-time pcr assay, these primers amplified only sequences present in c. jejuni to produce a product with a melting temperature of 81 ...200312929818
factors affecting the pressure resistance of some campylobacter species.to compare pressure resistance between strains of campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter fetus, and to investigate the effect of suspending medium on pressure resistance of sensitive and more resistant strains.200516162138
stable concentrated emulsions of the 1-monoglyceride of capric acid (monocaprin) with microbicidal activities against the food-borne bacteria campylobacter jejuni, salmonella spp., and escherichia coli.of 11 fatty acids and monoglycerides tested against campylobacter jejuni, the 1-monoglyceride of capric acid (monocaprin) was the most active in killing the bacterium. various monocaprin-in-water emulsions were prepared which were stable after storage at room temperature for many months and which retained their microbicidal activity. a procedure was developed to manufacture up to 500 ml of 200 mm preconcentrated emulsions of monocaprin in tap water. the concentrates were clear and remained stabl ...200616391087
thermophilic campylobacter spp. in danish broiler production: a cross-sectional survey and a retrospective analysis of risk factors for occurrence in broiler flocks.in order to elucidate the rate of thermophilic campylobacter spp. carriage in danish broiler production and to identify risk factors for occurrence of campylobacter in broiler flocks, a total of 88 randomly selected broiler flocks were tested for campylobacter infection, and a subsequent study of risk factors based on a questionnaire was conducted. the sample material comprised cloacal swabs from live birds before slaughter, and neck skin samples from carcasses at the end of the processing line. ...200019184798
prevalence of campylobacter spp. in raw retail poultry on sale in northern ireland.a year-long survey of fresh, retail poultry products on sale in northern ireland was undertaken to define the prevalence of campylobacter spp. by using protocols based on iso (standard) 10272-1:2006. incubation at 37 and 42 degrees c was undertaken to increase the diversity of isolates obtained. overall, 652 isolates were identified as campylobacter spp. by using pcr and amplified fragment length polymorphic typing. phenotyping wrongly identified 21% of isolates. prevalences of campylobacter fou ...200919777882
campylobacter species occurrence within internal organs and tissues of commercial caged leghorn laying hens.campylobacter spp. are frequently present in the intestinal tract and internal tissues of broiler breeder and broiler chickens. campylobacter spp. ecology in commercial leghorn laying hens has not been extensively studied. the objectives of the current study were to determine 1) campylobacter spp. presence in the reproductive tract, lymphoid organs, liver-gallbladder, and ceca of commercial leghorn laying hens; 2) species of campylobacter present; and 3) antimicrobial resistance pattern of campy ...200919834099
campylobacter spp among children with acute diarrhea attending mulago hospital in kampala--uganda.campylobacter infections occur worldwide. a recent study in kampala, uganda, found that 87% of broiler chickens had campylobacter jejuni; these are potential source of human infection. isolation rate in developing countries is between 5-35%. this study aimed at finding prevalence of children with campylobacter infection among children with acute diarrhea attending mulago hospital.200920589152
prevalence and antimicrobial resistance in campylobacter spp. isolated from retail chicken in two health units in ontario.campylobacter is an important enteric pathogen of humans and can cause diarrhea, fever, and abdominal pain. campylobacter infections have frequently been associated with the handling and consumption of raw and undercooked poultry. antimicrobial resistance among campylobacter strains is of concern in the treatment of campylobacteriosis in vulnerable populations. a 2-year multidisciplinary study was conducted in the perth and wellington-dufferin-guelph public health units in ontario, canada, to in ...201020615345
antimicrobial resistance of campylobacter isolates from retail meat in the united states between 2002 and 2007.the emergence of antimicrobial resistance in campylobacter spp. has been a growing public health concern globally. the objectives of this study were to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of campylobacter spp. recovered by the national antimicrobial resistance monitoring system (narms) retail meat program. retail meat samples (n = 24,566) from 10 u.s. states collected between 2002 and 2007, consisting of 6,138 chicken breast, 6,109 ground turkey, 6,171 ...201020971875
comparison of cape town and skirrow's campylobacter isolation protocols in humans and broilers in morogoro, tanzania.comparison of cape town and skirrow's protocols used in isolation of campylobacter in humans and broilers was carried out in a cross-sectional study in morogoro, tanzania. a total of 176 and 158 human stool and broiler intestinal samples were collected, respectively. while human stool samples were collected from selected health centers, broiler intestinal samples were obtained from selected farms and chicken markets. samples were inoculated and cultured in duplicate using two protocols and preva ...201121359592
eleutherine americana: a candidate for the control of campylobacter species.the antibacterial activity of ethanolic extracts of selected thai medicinal plants (rhodomyrtus tomentosa (aiton) hassk., quercus infectoria g. olivier, and eleutherine americana merr.) against campylobacter spp. was investigated. sixty-five campylobacter, including 39 isolates from humans and 26 isolates from chicken samples, were tested. reference campylobacter spp. that are commonly encountered in gastroenteritis were included. the ethanolic extract of e. americana demonstrated good antibacte ...201121406364
campylobacter contamination of broiler caeca and carcasses at the slaughterhouse and correlation with salmonella contamination.in order to estimate the prevalence of campylobacter spp. and salmonella spp. on broiler chicken carcasses and the prevalence of campylobacter spp. in caeca, 58 french slaughterhouses were investigated in 2008. enumeration of campylobacter spp. was also performed in order to study the relation between caeca and carcass contamination. a pool of 10 caeca and one carcass were collected from 425 different batches over a 12-month period in 2008. salmonella was isolated on 32 carcasses leading to a pr ...201121569927
rapid detection of campylobacter jejuni, campylobacter coli, and campylobacter lari in fresh chicken meat and by-products in bangkok, thailand, using modified multiplex pcr.a multiplex pcr assay for simultaneous detection and differentiation of campylobacter jejuni, campylobacter coli, and campylobacter lari was developed and validated to assess the occurrence of these bacteria in fresh chicken meat and by-products in bangkok, thailand, by using a new combination of four previously published pcr primers for c. jejuni, c. coli, c. lari, and a universal 16s rdna gene as an internal control. the specificity was determined by using 13 strains of other bacteria. with pu ...201526197289
prevalence of salmonella and campylobacter on broiler chickens from farm to slaughter and efficiency of methods to remove visible fecal contamination.a study was conducted to investigate the prevalence of salmonella and campylobacter from farm to slaughter. the efficiency of trimming and water spray (490 to 588 kpa pressure) on the removal of visible fecal contamination from broiler carcasses before chilling was also investigated. drag swabs were used to sample litter from the farm houses. samples of ceca and carcasses without and with visible fecal contamination before and after trimming or spray washing of fecal contamination were taken dur ...201425364917
prevalence and risk factors for thermotolerant species of campylobacter in poultry meat at retail in europe.the thermotolerant species campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis are the causative agents of the human illness called campylobacteriosis. this infection represents a threat for the health of consumers in europe. it is well known that poultry meat is an important food vehicle of campylobacter infection. as emerged from the reported scientific literature published between 2006 and 2016, poultry meat sold at retail level in europe represents an i ...201728854745
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