PMID(sorted ascending)
the development of a rapid sybr green i-based quantitative pcr for detection of duck circovirus.this report describes a one-step real-time polymerase chain reaction assay based on sybr green i for detection of a broad range of duck circovirus (ducv). align with all ducv complete genome sequences and other genus circovirus download from the genbank (such as goose circovirus, pigeon circovirus), the primers targets to the replicate gene of ducv were designed. the detection assay was linear in the range of 1.31 × 102-1.31 × 107 copies/μl. the reaction efficiency of the assay using the slope ( ...201121978576
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