structural and biological characteristics of reoviruses isolated from muscovy ducks (cairina moschata).two muscovy duck reoviruses, strains y1/79 and 1625/87, were investigated with regard to their genome organization, polypeptide pattern, serotype specificity, and pathogenicity. electrophoretic analysis of the genome revealed the migration pattern of avian reoviruses. in spite of general conformity, great polymorphism was detected in the electrophoretic mobility of individual genome segments of the two strains sharing only three segments of identical size (l1, m2, m3). only one segment (m3) migr ...199218670963
molecular cloning of peking duck toll-like receptor 3 (dutlr3) gene and its responses to reovirus infection.toll-like receptors (tlrs) play an important role in detecting pathogen-associated molecular patterns (pamps). among the tlrs, tlr3 is involved in the recognition of double-stranded rna. this study was designed to explore the relationship between dutlr3 and duck reovirus (drv) infection.201526634454
functional characterization of duck lsm14a in ifn-β induction.human lsm14a is a key component of processing body (p-body) assembly that mediates interferon-β (ifn-β) production by sensing viral rna or dna. to the best of our knowledge, we are the first to report duck lsm14a (dulsm14a) cloning from duck embryo fibroblasts (defs). full-length dulsm14a encoded 461 amino acids and was highly homologous with chicken and swan goose sequences. more interestingly, the dulsm14a mrna was extensively expressed in all the studied tissues. in defs, dulsm14a was localiz ...201728666650
complete sequence of a reovirus associated with necrotic focus formation in the liver and spleen of muscovy ducklings.the complete sequence of a reovirus, strain 815-12 associated with necrotic focus formation in the liver and spleen of muscovy ducklings in china, was determined and compared with sequences of other duck-, goose-, and chicken-origin reoviruses. the 815-12 genome comprised 22,969 bp with 10 dsrna segments ranging from 1125 bp (s4) to 3958 bp (l1), all of which (except s4) were almost identical in length to the cognate segments of other waterfowl and chicken isolates. detailed analyses revealed th ...201323820048
sequence and phylogenetic analysis of m-class genome segments of novel duck reovirus np03.we report the sequence and phylogenetic analysis of the entire m1, m2, and m3 genome segments of the novel duck reovirus (ndrv) np03. alignment between the newly determined nucleotide sequences as well as their deduced amino acid sequences and the published sequences of avian reovirus (arv) was carried out with dnastar software. sequence comparison showed that the m2 gene had the most variability among the m-class genes of drv. phylogenetic analysis of the m-class genes of arv strains revealed d ...201525852231
muscovy duck reovirus σns protein triggers autophagy enhancing virus replication.muscovy duck reovirus (mdrv) causes high morbidity and mortality in muscovy ducklings at 10 days old and can persist in an infected flock until the ducklings of 6 weeks old. it shares common physicochemical properties with avian reovirus (arv) and differs in coding assignment and pathogenicity. the arv p17 protein has been shown to trigger autophagy via activation multiple signaling pathways, which benefits virus replication. since mdrv lacks the p17 protein, whether and how mdrv induces autopha ...201728288679
isolation and genomic characterization of a classical muscovy duck reovirus isolated in zhejiang, china.a classical muscovy reovirus was isolated from a sick muscovy duck with white necrotic foci in its liver in zhejiang, china, in 2000. this classical reovirus was propagated in a chicken fibroblast cell line (df-1) with obvious cytopathic effects. its genome was 22,967 bp in length, with approximately 51.41% g+c content and 10 dsrna segments encoding 11 proteins, which formed a 3/3/4 electrophoretic page profile pattern. the length of the genomic segments was similar to those of avian orthoreovir ...201324140560
occurrence of reovirus infection in muscovy ducks (cairina moschata) in south western poland.during the summer 2012 an incidence of high mortality, above 44 percent, in two flocks of muscovy ducklings in poland was noted. the clinical signs included considerable weight loss and inability to walk. during the post-mortem evaluations dehydration and enteritis, gouty kidneys as well as hemorrhagic liver and spleen lesions were found. the laboratory diagnosis included agar gel precipitation assay (agp) as well as polymerase chain reaction (pcr) or reverse transcription pcr for the presence o ...201424988856
antigenic analysis of monoclonal antibodies against different epitopes of σb protein of avian reovirus.avian reovirus (arv) causes arthritis, tenosynovitis, runting-stunting syndrome (rss), malabsorption syndrome (mas) and immunosuppression in chickens. σb is one of the major structural proteins of arv, which is able to induce group-specific antibodies against the virus.201324312314
isolation and characterization of a reovirus causing spleen necrosis in pekin ducklings.high rates of mortality for pekin ducklings have been recorded in several duck farms in china since 2006. dead ducklings were characterized by spleen necrosis, suggesting microbial infection as a cause of disease. laboratory investigations led to the isolation of a virus strain from the spleen tissues of dead ducklings, designated drv-hc. subsequent experimental infections with drv-hc resulted in marked spleen necrosis in the ducklings similar to those observed in the natural outbreaks. electron ...201120970930
molecular characterization and expression of the s3 gene of muscovy duck reovirus strain 89026.although reovirus infection is one of the major virus diseases of muscovy ducks in france, no vaccine is available and nothing is known about the structure and function of the genes and proteins of the reovirus involved. the complete s3 genome segment of the muscovy duck reovirus strain 89026 has been cloned and the nucleotide and deduced amino acid sequences are reported here. the s3 genome segment is 1201 bp long and possesses the same terminal motifs (5' gcttttt and tattcatc 3') as the s3 gen ...19999934702
molecular characterization of a novel reovirus isolated from pekin ducklings in china.the complete genome sequence of a novel duck orthoreovirus, designated drv strain th11(drv-th11), was determined and characterized. the drv-th11 genome is comprised of 23,417 bp and its genome organization is more similar to that of avian orthoreoviruses (arvs) of chicken origin than other reoviruses. the results of comparative sequence analysis and dendrograms based on the µb- and σc-encoding genes indicated that th11 may be derived from the reassortment of arvs and classic muscovy duck reoviru ...201525287130
serological studies with reoviruses in chickens, turkeys and ducks.fluorescent antibody (fa) studies with avian reoviruses in chickens, turkeys and ducks are described. detection of the group-specific antigen by fa test was investigated by titrating a reovirus antiserum on chick embryo liver cell cultures infected with 18 reovirus strains fixed on multitest slides. with 16 of the viruses, test titres were similar, indicating presence of a common antigen. the titre observed with a duck reovirus isolate was considerably lower, suggesting partial cross-reactivity. ...19873119679
sequence analysis of the s3 gene from a turkey reovirus.the deduced sigma-2 protein sequence from the s3 gene segment of a novel turkey reovirus, designated nc98, isolated from the bursa of birds exhibiting poult enteritis and mortality syndrome was determined. the isolate, serologically distinct from other avian reoviruses, was isolated in turkey embryo kidney cells and rna was purified for cdna synthesis. oligonucleotide primers were designed based on conserved avian s3 nucleotide sequence data. the nc98 s3 open reading frame comprised 1,101 base p ...200212206313
Antigenic analysis monoclonal antibodies against different epitopes of sB protein of Muscovy duck reovirus.sB is one of the major structural proteins of Muscovy duck reovirus (DRV), which is able to induce protective immune response in target birds. Four anti-DRV sB MAbs were identified belong to two distinct epitopes, designated A (1E5, 4E3, and 5D8) and B (2F7) (Liu et al., 2010). To understand antigenic determinants of the sB protein, a set of 20 (P1-P20), partially overlapping and consecutive peptides spanning sB were expressed and then screened by MAbs. With Western blot and enzyme-linked immuno ...201122197425
genomic characteristics of a novel reovirus from muscovy duckling in china.a new reovirus was isolated from a sick muscovy duckling with hemorrhagic-necrotic lesions in the liver in zhejiang, china in 2000 and was tentatively denoted a new type of muscovy duck reovirus (n-mdrv zj00m). this reovirus was propagated in a chicken fibroblast cell line (df-1) with obvious cytopathic effects. the reovirus's genome was 23,419 bp in length with an approximately 50% g+c content and 10 dsrna segments encoding 12 proteins. the length of the genomic segments was similar to those of ...201424355531
muscovy duck reovirus sigmac protein is atypically encoded by the smallest genome segment.although muscovy duck reovirus (drv) shares properties with the reovirus isolated from chicken, commonly named avian reovirus (arv), the two virus species are antigenically different. similar to the drv sigmab-encoded gene (1201 bp long) previously identified, the three other double-stranded rna small genome segments of drv have been cloned and sequenced. they were 1325, 1191 and 1124 bp long, respectively, and contained conserved terminal sequences common to arvs. they coded for single expressi ...200211961275
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