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comparative evaluation of in-house manual, and commercial semi-automated and automated dna extraction platforms in the sample preparation of human stool specimens for a salmonella enterica 5'-nuclease assay.in the present study, three methods (nuclisens minimag [biomérieux], magna pure dna isolation kit iii bacteria/fungi [roche], and a silica-guanidiniumthiocyanate {si-guscn-f} procedure for extracting dna from stool specimens were compared with regard to analytical performance (relative dna recovery and down stream real-time pcr amplification of salmonella enterica dna), stability of the extracted dna, hands-on time (hot), total processing time (tpt), and costs. the si-guscn-f procedure showed th ...200717942177
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