| shared epitopes between mycoplasma pneumoniae major adhesin protein p1 and a 140-kilodalton protein of mycoplasma genitalium. | previous serological data have demonstrated cross-reactive antigens between two pathogenic species of mycoplasmas, m. pneumoniae and m. genitalium. preliminary analysis of sera and monoclonal antibodies (mabs) to protein antigens of these species showed an immunodominance of adhesin p1 (165 kilodaltons [kda]) of m. pneumoniae in mice and hamsters and a 140-kda protein of m. genitalium in mice and experimentally infected chimpanzees. to further characterize these two proteins, we assayed multiple ... | 1987 | 2432017 |
| superiority of the chimpanzee animal model to study the pathogenicity of known mycoplasma pneumoniae and reputed mycoplasma pathogens. | as far as we know, humans are the only known natural hosts for m. pneumoniae disease. whereas volunteer studies have provided useful data on the pathogenesis of disease and efficacy of vaccines, experimentally inducing disease in humans raises serious ethical questions and has become increasingly difficult to defend. thus, there is a genuine need for a satisfactory animal model to study m. pneumoniae disease. using the cotton rat and developing chick embryo models, eaton and co-workers (9-13) ha ... | 1987 | 3117729 |
| experimentally induced septic arthritis in chimpanzees infected with mycoplasma hominis, mycoplasma pneumoniae, and ureaplasma urealyticum. | mycoplasma hominis was isolated in pure culture from septic synovial aspirates from an individual (patient a) during 16 different bouts of exacerbation over a 70-month period of observation. two isolates, 10(7) and also 10(6) color-changing units (ccu) of the 1620 isolate and 5 x 10(4) ccu of the 1628 isolate, caused inflammation in chimpanzees inoculated intraarticularly. inflammation was also induced with 10(7) ccu of the 2010b isolate, serovar vii of ureaplasma urealyticum, recovered from an ... | 1994 | 8075258 |
| protection of immunized and previously infected chimpanzees challenged with mycoplasma pneumoniae. | following immunization, peak geometric mean serum metabolism inhibition antibody (mit) titres were 1:13 and 1:16 for groups of three chimpanzees each that received either the formalin-inactivated osu-1a or experimental acellular extract vaccine, respectively. following challenge, the mean titres for chimpanzees given the acellular vaccine peaked at 1:256 in 4 weeks and was 1:48 at 10 weeks. chimpanzees given the osu-1a vaccine peaked at 1:80 in 4 weeks and remained at 1:80 at 10 weeks. there was ... | 1994 | 8091848 |
| immunoblot analyses of chimpanzee sera after infection and after immunization and challenge with mycoplasma pneumoniae. | consecutive weekly or biweekly serum specimens obtained during a 3- or 4-month study from 16 chimpanzees were examined by immunoblot analyses to identify the immunogenic components of mycoplasma pneumoniae. six experimentally infected chimpanzees showed significant signs of overt disease, including cough, pharyngitis, rhinitis, fever, and loss of appetite. the sera of these infected chimpanzees recognized from 17 to 20 protein bands. two control chimpanzees that were not inoculated were included ... | 1994 | 8112834 |
| experimentally induced mycoplasma pneumoniae pneumonia in chimpanzees. | eight chimpanzees were examined. two served as negative control and six inoculated with mycoplasma pneumoniae became colonized. colonization persisted for 28-68, 16-50 and 21 days with an average duration of 47, 32.5 and 21 days in the oropharyngeal, tracheal and lung tissues, respectively. mycoplasma titers ranged from 10(8) to 10(1) color-changing units per specimen during the course of the infections. seroconversion occurred within 12-15 days and peak antibody titers ranged from 1.256 to 1.10 ... | 1993 | 8309353 |
| the immunodominant 90-kilodalton protein is localized on the terminal tip structure of mycoplasma pneumoniae. | immunoblot analysis of convalescent-phase sera of experimentally infected chimpanzees or monoclonal antibodies (mabs) specific to the 90- and 40-kda proteins of mycoplasma pneumoniae indicated that both proteins were present in cytadsorbing, pathogenic strains pi-1428, m129, and fh but absent in noncytadsorbing, nonpathogenic strain m129-b176. adsorption of convalescent-phase chimpanzee sera with virulent strain pi-1428 removed reactivity, whereas adsorption with avirulent strain m129-b176 did n ... | 1993 | 8454358 |
| models of mycoplasma respiratory and genital tract infections. | animal models for the study of human diseases must be replaced by in vitro methods, whenever possible. however, when critically evaluated, they remain indispensable for the solution of some specific problems in infectious diseases. these include the pathogenesis, the host response, as well as the study of antimicrobial agents and vaccines. under these conditions animal models, which closely reflect the situation in man, are especially valuable. two models may serve as "ideal" examples for the st ... | 1997 | 9286061 |