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partial identification of spirochaetes from two dairy cows with digital dermatitis by polymerase chain reaction analysis of the 16s ribosomal rna gene.specimens taken postmortem from typical lesions of digital dermatitis in two dairy cows were tested by the polymerase chain reaction (pcr) for the presence of a spirochaetal 16s rrna gene. seven different assays detected the gene in the samples from both cows. two of the pcr products were sequenced and a comparison of the nucleotide sequences revealed that the spirochaete belonged to the genus treponema and was closely related to treponema denticola. a pcr specific for the detection of the digit ...19979080644
the frequent detection of a treponeme in bovine digital dermatitis by immunocytochemistry and polymerase chain reaction.a study was carried out to determine whether spirochaetes are frequently associated with digital dermatitis in united kingdom (uk) dairy cattle. histopathological examination of lesions using a silver stain showed a large number of unidentified spirochaete-like organisms present in digital dermatitis hoof skin tissue in all examined biopsies. immunocytochemical staining demonstrated that spirochaetes in skin lesions were identified by polyclonal antisera to borrelia burgdorferi, treponema dentic ...19989646459
serological evidence of spirochaetal infections associated with digital dermatitis in dairy cattle.a potentially infectious aetiology for digital dermatitis in dairy cattle was investigated and centred on the possible involvement of spirochaetes. an enzyme-linked immunosorbent assay (elisa) was developed to detect bovine anti-borrelia burgdorferi (b31) and anti-treponeme (usa bovine isolates) antibodies in the sera of cows; sera were further tested for antigen specificity by western blotting. compared to normal cows, those with digital dermatitis had a much higher seropositivity rate to b. bu ...199910030131
detection of spirochetes by polymerase chain reaction and its relation to the course of digital dermatitis after local antibiotic treatment in dairy cattle.the aim of the study was to monitor the course of digital dermatitis after local antibiotic treatment in an experimental group (treated on diagnosis) and a control group (treated 5 days later). the present study was carried out on 2 farms involving 18 animals. monitoring was performed by means of clinical findings and detection of spirochetes on the surface of the lesions, using a polymerase chain reaction. superficial wound smears were taken before and after treatment. twelve animals on both fa ...199910216454
three unique groups of spirochetes isolated from digital dermatitis lesions in uk cattle.bovine digital dermatitis (bdd) is a severe infectious cause of lameness which has spread through dairy cattle populations worldwide, causing serious welfare and agricultural problems. spirochetes are the main organisms implicated and have previously proven difficult to isolate. this study aimed to isolate and characterise the range of spirochetes associated with bdd in the uk. twenty-three spirochete isolates were obtained from 30 bdd lesions, which by 16s rrna gene and flab2 gene analysis clus ...200818243592
altered microbiomes in bovine digital dermatitis lesions, and the gut as a pathogen reservoir.bovine digital dermatitis (dd) is the most important infectious disease associated with lameness in cattle worldwide. since the disease was first described in 1974, a series of treponema species concurrent with other microbes have been identified in dd lesions, suggesting a polymicrobial etiology. however, the pathogenesis of dd and the source of the causative microbes remain unclear. here we characterized the microbiomes of healthy skin and skin lesions in dairy cows affected with different sta ...201525781328
presence of digital dermatitis treponemes on cattle and sheep hoof trimming equipment.digital dermatitis (dd) is an infectious foot disease causing severe lameness in dairy cattle (worldwide) and sheep (uk). this study investigated whether dd treponema phylogroups can be present on equipment used to trim ruminant hooves and, therefore, consider this trimming equipment as a possible vector for the transmission of dd. equipment was tested after being used to trim dd symptomatic and asymptomatic cattle and sheep hooves, and subsequently after disinfection of equipment. after trimmin ...201424821857
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