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antibodies in horses, mules and donkeys following monovalent vaccination against african horse sickness.a total of 256 sera collected from three species of domesticated equidae in four different spanish provinces were examined 1-4 months after the administration of attenuated monovalent african horse sickness virus (ahsv) serotype 4 vaccine. approximately 10% of the sera were negative by elisa, virus neutralization, agar gel immuno-diffusion and complement fixation tests. similar negative reactions were recorded with sera from two ponies after experimental primary vaccination. the rapid rise in an ...19911902185
the spatial and seasonal distribution of african horse sickness and its potential culicoides vectors in morocco.african horse sickness (ahs) is a vector-borne, infectious disease of equines that is caused by african horse sickness virus (ahsv). the only proven field vector is the biting midge culicoides imicola, although c. obsoletus and c. pulicaris are suspected vectors. there was a recent epizootic of ahs in iberia (1987-90) and morocco (1989-91). in 1994-45 a total of 3887 light trap samples were taken from twenty-two sites distributed over most of morocco. culicoides imicola was found to be very wide ...19979330250
serological and virological responses in mules and donkeys following inoculation with african horse sickness virus serotype 4.two groups, comprising 4 donkeys and 4 mules (group 1) and 4 donkeys and 3 mules (group 2), were used to determine the duration of viraemia and to monitor the development of antibodies following inoculation with african horse sickness virus (ahsv). one group of animals was given a single dose of attenuated ahsv serotype 4 (ahsv 4) vaccine. the second group was inoculated with a virulent field strain of ahsv 4. both groups were subsequently challenged with the virulent field strain of ahsv 4, 51 ...19989785493
application of an indirect fluorescent antibody assay for the detection of african horse sickness virus antibodies.an indirect fluorescent antibody (ifa) technique was used to screen and quantify antibodies against african horse sickness virus (ahsv) in equine sera. results obtained with the ifa assay were compared directly with those obtained with standard complement fixation (cf) and virus neutralisation (vn) tests using horse sera from experimental studies and samples from the field. positive fluorescent antibody titres were detected from as early as 7 days after primary vaccination and persisted for at l ...19989785515
use of reverse transcriptase-polymerase chain reaction (rt-pcr) and dot-blot hybridisation for the detection and identification of african horse sickness virus nucleic acids.a coupled reverse transcriptase-polymerase chain reaction assay (rt-pcr) for the detection of african horse sickness virus (ahsv) dsrna, has been developed using genome segment 7 as the target template for primers. rna from isolates of all nine ahsv serotypes were readily detected. the potential inhibitory effects of either ethylene diamine tetra acetic acid (edta) or heparin on the rt-pcr were eliminated by washing blood samples before lysis of the red blood cells and storage. there was a close ...19989785517
serological survey of african horse sickness in selected districts of jimma zone, southwestern ethiopia.a cross-sectional serological survey was undertaken in selected districts of different agro-ecology of jimma zone (dedo, yebu, seka, serbo, and jimma town) from november 2009 to february 2010 to determine the seroprevalence of african horse sickness virus and associated risk factors of the disease. two hundred seventy-four equids (189 horses, 43 mules, and 47 donkeys) with a history of non-vaccination for at least 2 years were selected randomly from the above areas. sera samples were collected a ...201121465102
animal diseases caused by orbiviruses, algeria.antibodies against bluetongue virus were detected in cattle, sheep, goats, and camels in algeria in 2008. antibodies against epizootic hemorrhagic disease virus were detected in cattle, but antibodies against african horse sickness virus were not detected in horses and mules. epizootic hemorrhagic disease in northern africa poses a major risk for the european union.201122172371
outbreak investigation and molecular characterization of african horse sickness virus circulating in selected areas of ethiopia.the study was conducted from june 2011 to may 2012 in central, northern and western parts of ethiopia to investigate and identify circulating serotypes of african horse sickness virus (ahsv). the indigenous knowledge of equine owners about ahs in the study areas was assessed and also the retrospective data of ahs outbreaks for 2011 were analyzed. whole blood samples were collected for virus isolation and serotyping from diseased horses and mules showing typical signs of the ahs. virus isolation ...201323567554
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