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isolation and characterization of a novel betacoronavirus subgroup a coronavirus, rabbit coronavirus hku14, from domestic rabbits.we describe the isolation and characterization of a novel betacoronavirus subgroup a coronavirus, rabbit coronavirus hku14 (rbcov hku14), from domestic rabbits. the virus was detected in 11 (8.1%) of 136 rabbit fecal samples by reverse transcriptase pcr (rt-pcr), with a viral load of up to 10(8) copies/ml. rbcov hku14 was able to replicate in hrt-18g and rk13 cells with cytopathic effects. northern blotting confirmed the production of subgenomic mrnas coding for the he, s, ns5a, e, m, and n prot ...201222398294
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