| serologic evidence of brucella infection in pinnipeds along the coast of hokkaido, the northernmost main island of japan. | brucella infection in hokkaido was serologically surveyed in four species of pinnipeds inhabiting cape erimo during 2008-2013 and the shiretoko peninsula in 1999, by enzyme-linked immunosorbent assay using brucella abortus and b. canis as antigens. anti-brucella positive sera showed higher absorbance to b. abortus than b. canis in almost all samples. anti-b. abortus antibodies were detected in serum samples from 24% (n=55) of western pacific harbor seals (phoca vitulina stejnegeri) in cape erimo ... | 2017 | 28262966 |
| development, validation, and utilization of a competitive enzyme-linked immunosorbent assay for the detection of antibodies against brucella species in marine mammals. | a competitive enzyme-linked immunosorbent assay (celisa) was developed by using a whole-cell antigen from a marine brucella sp. isolated from a harbor seal (phoca vitulina). the assay was designed to screen sera from multiple marine mammal species for the presence of antibodies against marine-origin brucella. based on comparisons with culture-confirmed cases, specificity and sensitivity for cetacean samples tested were 73% and 100%, respectively. for pinniped samples, specificity and sensitivity ... | 2010 | 21088168 |
| seroconversion and abortion in cattle experimentally infected with brucella sp. isolated from a pacific harbor seal (phoca vitulina richardsi). | previously unrecognized brucella species have been isolated from a number of marine mammals, including harbor seals (phoca vitulina richardsi) in the puget sound area of the state of washington. because of the presence of dairy herds in proximity to the harbor seal populations, a study was conducted to determine the effects of the harbor seal brucella isolate in experimentally inoculated cattle. six pregnant cattle were exposed by intravenous injection (n = 3) or intraconjunctival inoculation (n ... | 2001 | 11580057 |