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molecular analysis of parapoxvirus from a spotted seal phoca largha in japan.a spotted seal phoca largha with nodular and scab lesions on the whole body was brought to an aquarium in nagoya, japan. we extracted dna from the lesions and used the polymerase chain reaction (pcr) method for detecting orthopoxvirus and parapoxvirus dna. parapoxvirus but not orthopoxvirus dna was detected. the partial nucleotide sequence of the envelope gene was determined from the pcr product, and the sequence was seen to be closely related to 2 parapoxvirus strains from spotted seals in alas ...201122235590
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