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lethal infection by a novel reassortant h5n1 avian influenza a virus in a zoo-housed tiger.in early 2013, a bengal tiger (panthera tigris) in a zoo died of respiratory distress. all specimens from the tiger were positive for hpai h5n1, which were detected by real-time pcr, including nose swab, throat swab, tracheal swab, heart, liver, spleen, lung, kidney, aquae pericardii and cerebrospinal fluid. one stain of virus, a/tiger/js/1/2013, was isolated from the lung sample. pathogenicity experiments showed that the isolate was able to replicate and cause death in mice. phylogenetic analys ...201525461468
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