| isolation of vibrio harveyi from diseased kuruma prawns penaeus japonicus. | outbreaks of high mortality among the cultured kuruma prawn penaeus japonicus without overt gross signs occurred during august and december of 1994 in i-lan, taiwan. eleven luminous bacterial strains were isolated from the hepatopancreas of moribund prawns from five different farms by use of tryptic soy agar (tsa, supplemented with 2% nacl) and/or thiosulfate citrate bile salt sucrose (tcbs) agar. these strains, together with our two previously unpublished isolates, were characterized and identi ... | 1996 | 8662185 |
| purification and characterization of a cysteine protease produced by pathogenic luminous vibrio harveyi. | the purification and characterization of an extracellular protease produced by pathogenic luminous vibrio harveyi strain 820514, originally isolated from diseased tiger prawn (penaeus monodon), was presented in this paper. the purification steps included ammonium sulfate precipitation, with columns of hydrophobic interaction chromatography and anion exchange on fast protein liquid chromatography. the protease is an alkaline cysteine protease, heat labile, inhibited by iodoacetamide, iodoacetic a ... | 1997 | 9175557 |
| passive immunization of the tiger prawn, penaeus monodon, using rabbit antisera to vibrio harveyi. | passive immunization, toxicity neutralization and the persistence of passive protection in the tiger prawn (penaeus monodon) were investigated using rabbit antisera to the formalinized extracellular products (ecp) (r alpha ecp) and/or formalinized bacterial cells (r alpha bc) of luminescent vibrio harveyi strain 820514 originally isolated from diseased tiger prawns. rabbit antiserum to bovine serum albumin (r alpha bsa) or phosphate-buffered saline (pbs, ph 7.2) both served as controls. the toxi ... | 1997 | 9248078 |
| cysteine protease is a major exotoxin of pathogenic luminous vibrio harveyi in the tiger prawn, penaeus monodon. | the role of an extracellular cysteine protease, produced by pathogenic luminous vibrio harveyi strain 820514 originally isolated from diseased tiger prawn (penaeus monodon), in the disease process in the prawns was studied. the protease was lethal to p. monodon with an ld50 value of 0.3 microgram protein g-1 prawn. the lethal toxicity of the extracellular products (ecp) of the bacterium was neutralized by pre-incubation of the ecp with rabbit antiserum to the cysteine protease. pre-incubation of ... | 1999 | 10389258 |
| hemostasis of tiger prawn penaeus monodon affected by vibrio harveyi, extracellular products, and a toxic cysteine protease. | the effects of bacterial cells, extracellular products (ecp) and a purified cysteine protease of vibrio harveyi on hemostasis and plasma components of tiger prawn (penaeus monodon) were studied. the clotting ability of the hemolymph withdrawn from moribund prawns pre-injected with the bacteria, ecp, cysteine protease of pbs (control) was observed for 2 h at 25 c. of these, only the control group was clottable while all the other groups were unclottable. a component of the plasma, previously iden ... | 1999 | 10575544 |
| electrophoretic characterization of a novel cysteine protease produced by vibrio harveyi. | electrophoretic characterization of a novel cysteine protease produced by pathogenic luminous vibrio harveyi, originally isolated from diseased tiger prawn penaeus monodon in taiwan, is demonstrated in the present study using native polyacrylamide gel electrophoresis (native page), sodium dodecyl sulfate-page (sds-page), crossed immunoelectrophoresis (cie) and isoelectric focusing (ief) gels. the protease has a pi of 6.4 and exhibits a fast-migrating feature in native-page and cie gels indicatin ... | 1999 | 10608698 |
| inhibition of luminescence and virulence in the black tiger prawn (penaeus monodon) pathogen vibrio harveyi by intercellular signal antagonists. | expression of luminescence in the penaeus monodon pathogen vibrio harveyi is regulated by an intercellular quorum sensing mechanism involving the synthesis and detection of two signaling molecules, one of which is n-hydroxy butanoyl-l-homoserine lactone and the other of which is uncharacterized. indirect evidence has suggested that virulence, associated with a toxic extracellular protein, and luminescence in v. harveyi are coregulated. in this study the effects of an acylated homoserine lactone ... | 2000 | 10788385 |
| a beta-1,3-glucan binding protein from the black tiger shrimp, penaeus monodon. | a beta-1,3-glucan binding protein (gbp) has been isolated from a shrimp hemocyte cdna library. its open reading frame consists of 1314 nucleotides with a polyadenylated sequence and a poly a tail. it encodes a polypeptide of 370 amino acids including a 17 amino acid-signal peptide. the mature protein has an estimated molecular mass of 39.5 kda and a predicted pi of 5.5. sequence comparison shows a high degree of similarity to invertebrate recognition proteins with glucanase-like domains for exam ... | 2002 | 11755673 |
| control of pathogenic vibrio spp. by bacillus subtilis bt23, a possible probiotic treatment for black tiger shrimp penaeus monodon. | the present study evaluated the in vitro and in vivo antagonistic effect of bacillus against the pathogenic vibrios. | 2003 | 12535126 |
| induction of peroxide and superoxide protective enzymes and physiological cross-protection against peroxide killing by a superoxide generator in vibrio harveyi. | vibrio harveyi is a causative agent of destructive luminous vibriosis in farmed black tiger prawn (penaeus monodon). v. harveyi peroxide and superoxide stress responses toward elevated levels of a superoxide generated by menadione were investigated. exposure of v. harveyi to sub-lethal concentrations of menadione induced high expression of genes in both the oxyr regulon (e.g., a monofunctional catalase or kata and an alkyl hydroperoxide reductase subunit c or ahpc), and the soxrs regulon (e.g., ... | 2003 | 12694915 |
| enhanced growth and resistance to vibrio challenge in pond-reared black tiger shrimp penaeus monodon fed a bacillus probiotic. | the bacterial probiont bacillus s11 (bs11) was used as a supplement in feed (pf) for black tiger shrimp penaeus monodon in 2 earthen pond field-trials carried out for 100 d during 2 different seasons in thailand. growth and survival were compared with those of shrimp receiving an unsupplemented feed (uf). in the hot and cool seasons, respectively, shrimp fed pf grew significantly larger and had significantly higher survival than shrimp fed uf (p < 0.05). projected yields on an annual basis (two ... | 2003 | 12911065 |
| antimicrobial peptides discovered in the black tiger shrimp penaeus monodon using the est approach. | two cdna libraries were prepared from hemocytes of normal and vibrio harveyi-challenged black tiger shrimp penaeus monodon. a total of 1062 expressed sequence tag (est) clones were sequenced unidirectionally. ests representing the antimicrobial peptide (amp) homologues, antilipopolysaccharide factors (alf), penaeidins and crustins were discovered. they predominated among immune-related genes, representing 29.2% and 64.0% of the normal and challenged libraries, respectively. several types of each ... | 2004 | 15584419 |
| bacterial clearance rate and a new differential hemocyte staining method to assess immunostimulant activity in shrimp. | new methods were developed to assess immunostimulant efficacy in the black tiger shrimp penaeus monodon. test shrimp were fed with 2 or 4 % yeast extract (ye)-coated feed while controls were fed non-coated feed. after 4 wk of feeding, individual shrimp were assessed for total hemocyte counts (thc), the number of granular hemocytes (gh) and rate of bacterial clearance. for hemocyte counts, formalin-fixed hemolymph was stained with 1.2 % rose bengal in 50 % ethanol for 20 min at room temperature. ... | 2005 | 15759805 |
| molecular cloning and characterization of cdna of penaeidin-like antimicrobial peptide from tiger shrimp (penaeus monodon). | we report the molecular cloning and characterization of a penaeidin-like antimicrobial peptide (amp) complementary dna from the hemocytes of tiger shrimp (penaeus monodoni). a tiger shrimp amp cdna containing the entire coding region of the peptide, determined by rapid amplification of cdna 5' and 3' ends and polymerase chain reaction of the messenger rna isolated from hemocytes of the shrimp, is 683 bp in length with an open reading frame of 222 bp. the deduced amino acid sequence of this antim ... | 2005 | 15776314 |
| production of monoclonal antibodies for detection of vibrio harveyi. | monoclonal antibodies (mabs) against vibrio harveyi were produced from mice immunized with heat-killed and sds-mercaptoethanol-treated highly virulent v. harveyi 639. fifteen mabs were selected and sorted into 6 groups according to their specificity to various proteins of apparent molecular weight ranging from 8 to 49 kda. some antibodies were used for detection of v. harveyi at concentrations as low as 10(4) cfu ml(-1) using immunodot blots. most of the selected mabs did not show cross-reactivi ... | 2005 | 15819431 |
| partial characterization of a novel bacteriophage of vibrio harveyi isolated from shrimp culture ponds in thailand. | a bacteriophage was isolated together with vibrio harveyi (vh) 1114 a from a black tiger shrimp-rearing pond in thailand. by negative staining transmission electron microscopy (tem), the phage had an icosahedral head (diameter 60-62 nm), a rigid, non-contractile tail (9-10 nm x 100-120 nm) without a collar or terminal fibers and a genome of double stranded dna of approximately 80 kb as determined by analysis of restriction enzyme digestion fragments. since these features would place it in the fa ... | 2005 | 16023243 |
| phenotypic and molecular typing of vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae. | the objective of the present study was to identify the biotype(s) and molecular type(s) of vibrio harveyi associated with pathogenicity in tiger shrimp (penaeus monodon) larvae. | 2006 | 17032233 |
| molecular cloning, characterization and expression of a masquerade-like serine proteinase homologue from black tiger shrimp penaeus monodon. | a full-length cdna of a masquerade-like serine proteinase homologue (pmmassph) of penaeus monodon was cloned and characterized by rapid amplification cdna end (race) method. the complete cdna sequence of 1958bp contains an open reading frame (orf) of 1572bp, encoding a 523 amino acid protein including a 19 amino acid signal peptide. the calculated molecular mass of the mature protein (504 amino acids) is 51.58kda with an estimated pi of 4.86. pmmassph has most of the structural characteristics o ... | 2007 | 17174571 |
| molecular cloning, genomic organization and recombinant expression of a crustin-like antimicrobial peptide from black tiger shrimp penaeus monodon. | a novel crustin-like antimicrobial peptide (crus-likepm) was identified from haemocytes of penaeus monodon. the deduced amino acid sequence of a crus-likepm consists of 124 amino acid residues of the mature peptide and a signal peptide of 17 amino acid residues. the mature peptide contains a glycine-rich domain at the n-terminus and 12 conserved cysteine residues containing a single wap domain at the c-terminus. phylogenetic tree and sequence comparison clearly confirmed a distinct between a cru ... | 2008 | 17850873 |
| anti-lipopolysaccharide factors from the black tiger shrimp, penaeus monodon, are encoded by two genomic loci. | different isoforms of the alf homologues (alfpm1-5) have been previously identified from penaeus monodon expressed sequence tag (est) database (http://pmonodon.biotec.or.th). the nucleotide and amino acid sequences of the p. monodon alf homologues were analyzed and categorized into two groups, alfpm1 and 2 in group a and alfpm3-5 in group b. the genomic sequences of the two alf gene groups were obtained by using the pcr and genome walking techniques. the alf group a gene consisted of three exons ... | 2008 | 18083045 |
| the cycle for a siphoviridae-like phage (vhs1) of vibrio harveyi is dependent on the physiological state of the host. | in a previous report, we isolated vibrio harveyi (vh) 1114 together with its bacteriophage, vhs1, from a black tiger shrimp-rearing pond. the vhs1 has its lysogenic relationship to the vh1114 host as either true lysogen (tl) or pseudolysogen (pl). the characters of tl are based on the extrachromosomal existence of the vhs1 phage genome in the vh host which also simultaneously produces the vhs1 phage particles and is resistant to super-infection. the original vh1114 host exhibits a clear plaque a ... | 2008 | 18448184 |
| localization of anti-lipopolysaccharide factor (alfpm3) in tissues of the black tiger shrimp, penaeus monodon, and characterization of its binding properties. | anti-lipopolysaccharide factor (alf) is an antimicrobial peptide originally identified from horseshoe crabs and recently found in several shrimp species. alfpm3, the most abundant isoform in the black tiger shrimp, penaeus monodon, has been shown to possess a broad spectrum of antimicrobial activity against gram-negative and gram-positive bacteria, and filamentous fungi. in this study, a potential role for alfpm3 in the shrimp innate immunity was revealed by examining the distribution of the pro ... | 2008 | 18466970 |
| identification of immune-related genes from kidney and spleen of turbot, psetta maxima (l.), by suppression subtractive hybridization following challenge with vibrio harveyi. | suppression subtractive hybridization was used to investigate the response of turbot, psetta maxima (l.), to vibrio harveyi, by using a cdna library constructed from artificially infected turbot kidney and spleen mrna. forty-nine expressed sequence tags were obtained. several immune system genes were identified, including a major histocompatibility complex (mhc) class ia gene and a heat shock protein 70 gene. some signalling molecules were also present in the cdna libraries, including src-family ... | 2008 | 18577100 |
| abundantly expressed transcripts in the lymphoid organ of the black tiger shrimp, penaeus monodon, and their implication in immune function. | the lymphoid organ of penaeid shrimps is proposed to play an important role in the innate immune system. to investigate the potential immune function of the lymphoid organ, we analyzed the expressed genes from the lymphoid organ of normal and vibrio harveyi-infected penaeus monodon using an expressed sequence tag (est) approach. sequence analysis of the est clones derived from the two lymphoid organ cdna libraries (408 clones from the normal and 625 clones from the infected libraries), revealed ... | 2008 | 18692576 |
| two prophenoloxidases are important for the survival of vibrio harveyi challenged shrimp penaeus monodon. | phenoloxidase (po) plays an important role in arthropod melanization. previously, a prophenoloxidase (pmpropo1) gene was cloned and characterized from the hemocytes of the black tiger shrimp, penaeus monodon. in the present study, we report a novel propo gene (pmpropo2) belonging to the propo family identified from the p. monodon est database (http://pmonodon.biotec.or.th). the full-length sequence of pmpropo2 consists of 2513bp encoding a predicted 689 amino acid residues with a calculated mole ... | 2009 | 18834900 |
| domain inhibitory and bacteriostatic activities of the five-domain kazal-type serine proteinase inhibitor from black tiger shrimp penaeus monodon. | serine proteinase inhibitors (spis) in multi-cellular organisms are important modulators of proteinase activities in various biological processes. a five-domain kazal-type spi spipm2 from the black tiger shrimp penaeus monodon is presumably involved in innate immune response. the spipm2 with the domain p1 residues t, a, e, k and e was isolated from the hemocyte cdna libraries and found to strongly inhibit subtilisin and elastase, and weakly inhibit trypsin. to unravel further the inhibitory acti ... | 2009 | 18930077 |
| functional characterization of a masquerade-like serine proteinase homologue from the black tiger shrimp penaeus monodon. | a cdna encoding a masquerade-like serine proteinase homologue (pmmassph) from the black tiger shrimp, penaeus monodon, has been cloned and characterized. the transcript of pmmassph is induced in response to vibrio harveyi infection. to further characterize the function(s) of the protein, (i) the n-terminal region comprising the glycine-rich repeats and the clip domain, and (ii) the c-terminal sp-like domain of the pmmassph were separately cloned into the pet-28b(+) expression vector and transfor ... | 2009 | 19328243 |
| gene silencing of a prophenoloxidase activating enzyme in the shrimp, penaeus monodon, increases susceptibility to vibrio harveyi infection. | the prophenoloxidase (propo) activating system is an important innate immune response against microbial infections in invertebrates. the major enzyme, phenoloxidase (po), is synthesized as an inactive precursor and its activation to an active enzyme is mediated by a cascade of clip domain serine proteinases (clip-sps). in this study, a cdna encoding a propo activating enzyme (ppae) from the black tiger shrimp, penaeus monodon, designated as pmppae1, was cloned and characterized. the full-length ... | 2009 | 19428482 |
| kazal-type serine proteinase inhibitors from the black tiger shrimp penaeus monodon and the inhibitory activities of spipm4 and 5. | serine proteinase inhibitors (spis) play important roles in physiological and immunological processes involving proteinases in all multicellular organisms. in black tiger shrimp penaeus monodon, nine different kazal-type spis, namely spipm1-9, were identified from the cdna libraries of hemocyte, hepatopancreas, hematopoietic tissue, ovary and lymphoid organ. they are multi-domain spis containing 2-7 and possibly more kazal domains. two interesting cdna clones, spipm4 and spipm5 coding for two-do ... | 2009 | 19497371 |
| expression of immune-related genes in the digestive organ of shrimp, penaeus monodon, after an oral infection by vibrio harveyi. | in all previous studies, to study shrimp immune response, bacteria were directly injected into the shrimp body and as a consequence the initial step of a natural interaction was omitted. in this study we have instead used an immersion technique, which is a more natural way of establishing an infection, to study immune responses in black tiger shrimp (penaeus monodon). normally, vibrio harveyi (vh) is highly pathogenic to post-larval shrimp, but not to juveniles which usually resist an infection. ... | 2010 | 19646472 |
| studies on the immunomodulatory effect of polysaccharide gel extracted from durio zibethinus in penaeus monodon shrimp against vibrio harveyi and wssv. | oral administration of polysaccharide gel (pg) in shrimp diets revealed immunostimulating potential and disease resistance in penaeus monodon (black tiger shrimp). pg from the fruit-rind of durio zibethinus has been characterized to be a pectic polysaccharide with immunomodulating and antibacterial activities. pg inhibited growth of the shrimp bacterial pathogen, vibrio harveyi 1526, by agar diffusion and broth microdilution tests. clear inhibition zones on agar plates were observed at the lowes ... | 2010 | 20034573 |
| recombinant ferritin protein protects penaeus monodon infected by pathogenic vibrio harveyi. | hemocytes of shrimp perform an essential role in defense against microbial pathogens, involving both cellular and humoral factors. the gene coding for ferritin in black tiger shrimp penaeus monodon was cloned, sequenced and expressed using pqe-30-ua vector and sg13009 escherichia coli host cells. the deduced amino acid sequence of p. monodon ferritin showed 32 to 95% similarity with ferritin proteins of other organisms. the recombinant protein was purified by nickel-nitrilotriacetic acid affinit ... | 2010 | 20225671 |
| expression and distribution of three heat shock protein genes under heat shock stress and under exposure to vibrio harveyi in penaeus monodon. | a sudden increase in temperature results in heat shock stress of the cultured shrimp. to cope with the stress, shrimp has to overcome by triggering a response known as heat shock response. to understand the heat shock response in the black tiger shrimp (penaeus monodon), we examined expression patterns and distribution of three heat shock protein (hsp) genes in p. monodon juveniles. the expression levels of hsp21, hsp70 and hsp90 were determined by quantitative real-time pcr in nine tissues (gil ... | 2010 | 20561967 |
| cloning and characterization of a melanization inhibition protein (pmmip) of the black tiger shrimp, penaeus monodon. | melanization is an important component of the innate immune responses in invertebrates and it is essential for defense against invading microorganism. melanin formation, which is a result of activation of the so called prophenoloxidase activating system, needs to be controlled due to the dangerous effects of quinones and melanin which are produced during the process of melanization. here, a cdna for a melanization inhibition protein (mip), named pmmip, was identified from the black tiger shrimp, ... | 2010 | 20561997 |
| histopathological studies on vibrio harveyi- infected tiger puffer, takifugu rubripes (temminck et schlegel), cultured in japan. | vibrio harveyi infection occurred with a moderate mortality in tiger puffer, takifugu rubripes (temminck et schlegel), in autumn 2007, at a mariculture farm in western japan. the diseased fish showed nodular lesions in the branchial chamber and the inner surface of the operculum. histopathologically, the lesions comprised granulation tissue containing many suppurative foci allowing propagation of the bacteria and granuloma encapsulating abscesses with a decrease in bacteria. the bacteria were di ... | 2010 | 20726939 |
| pmppae2, a new class of crustacean prophenoloxidase (propo)-activating enzyme and its role in po activation. | the prophenoloxidase (propo) activating system plays an important role in the defense against microbial invasion in invertebrates. in the present study, we report a second propo-activating enzyme (designated pmppae2) from the hemocytes of the black tiger shrimp, penaeus monodon. pmppae2 contained the structural features of the clip domain serine proteinase family and exhibited 51% amino acid sequence similarity to the insect manduca sexta pap-1. amino acid sequence alignment with the available a ... | 2011 | 20837056 |
| solvent extracts of the red seaweed gracilaria fisheri prevent vibrio harveyi infections in the black tiger shrimp penaeus monodon. | vibriosis is a common bacterial disease that can cause high mortality and morbidity in farmed shrimp. since compounds from seaweed have been reported to have anti-bacterial and immunostimulant activity, this study was conducted to determine whether solvent extracts from the red seaweed gracilaria fisheri might be a possible alternative for prevention and treatment of shrimp vibriosis caused by vibrio harveyi. seaweed extracts prepared using ethanol, methanol, chloroform and hexane were evaluated ... | 2010 | 21115128 |
| a double wap domain-containing protein pmdwd from the black tiger shrimp penaeus monodon is involved in the controlling of proteinase activities in lymphoid organ. | a homolog of mammalian secretory leucocyte proteinase inhibitor or slpi known as a double wap domain (dwd) protein has been found in penaeid shrimp and believed to play an important role in innate immune system of the shrimp. the pmdwd identified from the penaeus monodon est database was investigated for its expression under pathogen infection. infections by vibrio harveyi and white spot syndrome virus (wssv) up-regulated the expression of the pmdwd, which was peaked at about 24 h post infection ... | 2011 | 21216291 |
| A comparative study of antimicrobial properties of crustinPm1 and crustinPm7 from the black tiger shrimp Penaeus monodon. | Several isoforms of crustin have been identified in the black tiger shrimp Penaeus monodon. These cationic cysteine-rich antimicrobial peptides contain a single whey acidic protein (WAP) domain at the C-terminus and exhibit antimicrobial activity against both Gram-positive and Gram-negative bacteria. In this paper, we investigate the binding properties and antimicrobial actions of crustinPm1 and crustinPm7, the two most abundant crustin isoforms found in the haemocyte of P. monodon. Previously, ... | 2012 | 21855569 |
| shrimp serine proteinase homologues pmmassph-1 and -2 play a role in the activation of the prophenoloxidase system. | melanization mediated by the prophenoloxidase (propo) activating system is a rapid immune response used by invertebrates against intruding pathogens. several masquerade-like and serine proteinase homologues (sphs) have been demonstrated to play an essential role in propo activation in insects and crustaceans. in a previous study, we characterized the masquerade-like sph, pmmassph1, in the black tiger shrimp penaeus monodon as a multifunctional immune protein based on its recognition and antimicr ... | 2015 | 25803442 |
| a novel c-type lectin in the black tiger shrimp penaeus monodon functions as a pattern recognition receptor by binding and causing bacterial agglutination. | c-type lectins are pattern recognition proteins that play important roles in innate immunity in invertebrates by mediating the recognition of pathogens. in this study, a novel c-type lectin gene, pmclec, was cloned and characterized from the black tiger shrimp penaeus monodon. the open reading frame of pmclec is 657 bp in length. it encodes a predicted protein of 218 amino acids with a calculated molecular mass and an isoelectric point of 24086 da and 4.67, respectively. sequence analysis of pmc ... | 2017 | 27876622 |
| melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens. | melanization is a rapid defense mechanism in invertebrates. the substrate specificity of phenoloxidases (pos) and the role of melanization reaction products were investigated in the black tiger shrimp, penaeus monodon. two pmpos (pmpropo1 and pmpropo2) were found to display a substrate specificity towards monophenols and diphenols, and exhibit relatively weak activity against 5,6-dihydroxyindole (dhi). systemic infection of the pmpropo1/2 co-silenced shrimp with the fungus, fusarium solani, led ... | 2014 | 25043262 |
| gene expression and characterization of a serine proteinase inhibitor pmserpin8 from the black tiger shrimp penaeus monodon. | the ubiquitous serpins or serine proteinase inhibitors are essential for controlling proteinases in several biological processes in various organisms. a pmserpin8, one of eight serpins identified from the penaeus monodon database, is studied and reported herein. the open reading frame of pmserpin8 gene derived from a genomic gene contains 5 exons of 320, 139, 244, 239 and 312 bp separated by 4 introns of 447, 657, 326 and 479 bp. the pmserpin8 gene is highly expressed at nauplius stage and gradu ... | 2012 | 22738762 |
| melanization inhibition protein of penaeus monodon acts as a negative regulator of the prophenoloxidase-activating system. | melanization mediated by the prophenoloxidase-activating system (propo) is an important immune response in invertebrates. however, in addition to melanin, the propo system produces reactive intermediates that are not only harmful to the invading microbes but also to the host cells. thus, the propo system must be tightly regulated by several inhibitors. previously, a melanization inhibition protein from the black tiger shrimp penaeus monodon, pmmip, has been identified and preliminarily character ... | 2017 | 28232130 |
| pmserpin3 from black tiger shrimp penaeus monodon is capable of controlling the propo system. | serpin or serine proteinase inhibitor is a family of protease inhibitors that are involved in controlling the proteolytic cascade in various biological processes. in shrimp, several serpins have been identified but only a few have been characterized. herein, the pmserpin3 gene identified from penaeus monodon est database was studied. by using the 5'- and 3'-rapid amplification of cdna ends (race) techniques, the full-length of pmserpin3 cdna was obtained. the cdna contained an open reading frame ... | 2013 | 23660411 |
| characterization and expression analysis of a chitinase gene (pmchi-4) from black tiger shrimp (penaeus monodon) under pathogen infection and ambient ammonia nitrogen stress. | chitinase is a multi-gene family, which play important physiological roles in crustaceans, involved in several biological processes, including digestion, molting and defense against viruses. in the present study, a chitinase-4 gene (pmchi-4) was cloned from penaeus monodon by rapid amplification of cdna ends (race). the full length of pmchi-4 cdna was 2178 bp, including an 1815 bp open reading frame (orf) which encoded 604 amino acid residues. the predicted pmchi-4 protein was 67.7 kda and share ... | 2017 | 28089896 |
| the immune response of the c-jun in the black tiger shrimp (penaeus monodon) after bacterial infection. | the transcription factor c-jun widely exists in vertebrates and invertebrates and plays an important role in various kinds of stimulus response. in this study, pmc-jun gene was first cloned from penaeus monodon. the full-length cdna of pmc-jun was 1857 bp in length and included an 879 bp open reading frame (orf), which encoded 293 amino acids. qrt-pcr analysis results showed that pmc-jun mrnas were ubiquitously expressed in all the examined tissues. the highest expression level was observed in g ... | 2017 | 28027988 |
| improvement of innate immune responses and defense activity in tiger shrimp (penaeus monodon fab.) by intramuscular administration of the outer membrane protein vibrio alginolyticus. | the outer membrane protein (omp) of vibrio alginolyticus cell wall was administered intramuscularly (im) to the tiger shrimp (penaeus monodon fab.) at 10, 20, 30 μg/kg bw. after 14 days infection, the tiger shrimps were challenged with 10(7) bacterial density of vibrio harveyi for 24 hours. the total haemocyte count (thc), differential haemocyte count (dhc) and amount of total protein plasma (tpp), superoxide dismutase and protease enzyme activity were monitored. the results showed that intramus ... | 2013 | 24058892 |
| antibacterial function of herbal extracts on growth, survival and immunoprotection in the black tiger shrimp penaeus monodon. | this study examined the effects of an herbal extract composed of nine herbs i.e aloe vera, andrographis pariculata, annona squamosa, azadirachta indica, citrus aurantifolia, coriandrum sativum, ocimum sanctum, ollium cepa and psidium guajava on growth, survival rate and immunoprotection against pathogenic vibrio harveyi in the tiger shrimp penaeus monodon. the petroleum ether, methanol and n-hexen extracts of different herbal plants were selected, processed and thoroughly mixed in equal proporti ... | 2017 | 28365386 |
| a snake-like serine proteinase (pmsnake) activates prophenoloxidase-activating system in black tiger shrimp penaeus monodon. | clip domain serine proteinases (clipsps) play critical roles in the activation of proteolytic cascade in invertebrate immune systems including the prophenoloxidase (propo) activating system. in this study, we characterized a snake-like serine protease, namely pmsnake, from the shrimp penaeus monodon which has previously been identified based on the subtractive cdna library of propo double-stranded rna (dsrna)-treated hemocytes. an open reading frame of pmsnake contains 1068 bp encoding a predict ... | 2017 | 27693192 |
| cloning, characterization, and expression of the macrophage migration inhibitory factor gene from the black tiger shrimp (penaeus monodon). | macrophage migration inhibitory factor (mif) is an ancient cytokine that engages in innate immune system of vertebrates and invertebrates. in this study, the mif gene homologue (pmmif) was cloned from the black tiger shrimp, penaeus monodon. the full-length cdna sequence of pmmif was 838 bp and contained 78 bp 5' untranslated region (utr) and 397 bp 3' utr, and an open reading frame (orf) of 363 bp which coded 120 amino acids (aa). multiple alignment analysis showed that the deduced amino acid s ... | 2016 | 27514787 |
| molecular characterization of antibiotic resistant vibrio harveyi isolated from shrimp aquaculture environment in the south east coast of india. | vibrio harveyi is a strategic human pathogen that occurs naturally in marine and estuarine environments. the pathogen is believed to cause acute septicemia, gastroenteritis, severe necrotizing soft-tissue infection, and high rate of lethality through ingestion of v. harveyi contaminated seafood. in this study, we isolated and characterized v. harveyi from water suspended sediment samples of black tiger shrimp ponds and from the sea coasts, in the east coast of the bay of bengal, india. initial i ... | 2016 | 27247095 |
| bacterial dynamics in intestines of the black tiger shrimp and the pacific white shrimp during vibrio harveyi exposure. | the intestinal microbiota play important roles in health of their host, contributing to maintaining the balance and resilience against pathogen. to investigate effects of pathogen to intestinal microbiota, the bacterial dynamics upon a shrimp pathogen, vibrio harveyi, exposures were determined in two economically important shrimp species; the black tiger shrimp (bt) and the pacific white shrimp (pw). both shrimp species were reared under the same diet and environmental conditions. shrimp surviva ... | 2016 | 26585302 |
| a shrimp pacifastin light chain-like inhibitor: molecular identification and role in the control of the prophenoloxidase system. | pacifastin is a recently classified family of serine proteinase inhibitors that play essential roles in various biological processes, including in the regulation of the melanization cascade. here, a novel pacifastin-related gene, termed pmpacifastin-like, was identified from a reverse suppression subtractive hybridization (ssh) cdna library created from hemocytes of the prophenoloxidase pmpropo1/2 co-silenced black tiger shrimp penaeus monodon. the full-length sequences of pmpacifastin-like and ... | 2016 | 26271600 |
| characterization and identification of calmodulin and calmodulin binding proteins in hemocyte of the black tiger shrimp (penaeus monodon). | calmodulin (cam), a ubiquitous intracellular calcium (ca(2+)) sensor in all eukaryotic cells, is one of the well-known signaling proteins. previously, cam gene has shown a high transcriptional level in hemocyte of the pathogen infected shrimp, suggesting that shrimp cam does not only regulate ca(2+) metabolism, but is also involved in immune response cascade. in the present study, the cam gene of shrimp penaeus monodon was identified and the recombinant p.monodon cam (rpmcam) was produced and bi ... | 2015 | 25681078 |
| determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. | a new emerging disease in shrimp, first reported in 2009, was initially named early mortality syndrome (ems). in 2011, a more descriptive name for the acute phase of the disease was proposed as acute hepatopancreatic necrosis syndrome (ahpns). affecting both pacific white shrimp penaeus vannamei and black tiger shrimp p. monodon, the disease has caused significant losses in southeast asian shrimp farms. ahpns was first classified as idiopathic because no specific causative agent had been identif ... | 2013 | 23836769 |
| application of bacterial lipopolysaccharide to improve survival of the black tiger shrimp after vibrio harveyi exposure. | this study investigates an effect of bacterial lipopolysaccharide (lps) as feed supplement to improve immunity of the black tiger shrimp (penaeus monodon). lps was coated to commercial feed pellets and given to the shrimp once or twice a day for 10 days before an exposure with shrimp pathogenic bacterium vibrio harveyi. the growth rates, percent weight gains, total hemocyte and granulocyte counts and survival rates of shrimp between the lps-coated pellet fed groups and a control group where shri ... | 2013 | 23751331 |
| complete genome sequence of virulence-enhancing siphophage vhs1 from vibrio harveyi. | vibrio harveyi siphophage 1 (vhs1) is a tailed phage with an icosahedral head of approximately 66 nm in diameter and an unornamented, flexible tail of approximately 153 nm in length. when vibrio harveyi 1114gl is lysogenized with vhs1, its virulence for the black tiger shrimp (penaeus monodon) increases by more than 100 times, and this coincides with production of a toxin(s) associated with shrimp hemocyte agglutination. curiously, the lysogen does not show increased virulence for the whiteleg s ... | 2012 | 22307287 |
| screening and evaluation of local bacteria isolated from shellfish as potential probiotics against pathogenic vibrios. | the present study was carried out to isolate, screen and evaluate potential candidates of local bacteria isolated from tiger shrimp penaeus monodon and slipper cupped oysters crassostrea iredalei as probiotics in shellfish aquaculture. a total of 144 of bacteria were successfully isolated from the intestine and stomach of 20 tails of healthy adult tiger shrimp p. monodon, while 136 were successfully isolated from the digestive tract, gills and inner shells of 10 healthy adult c. iredalei. the nu ... | 2016 | 28779740 |
| gene characteristics, immune and stress responses of pmprx1 in black tiger shrimp (penaeus monodon): insights from exposure to pathogenic bacteria and toxic environmental stressors. | peroxiredoxins (prxs) are ubiquitous, multifunctional and evolutionarily conserved enzymes that can protect cells from oxidative damage caused by ros and play a vital role in immune responses. here, a full-length prx1 cdna sequence (pmprx1) was isolated from penaeus monodon. the pmprx1 cdna was 951 base pairs (bp), encoding 198 amino acid polypeptides. the results of qrt-pcr showed that the pmprx1 mrna was ubiquitously expressed in all tissues tested and had a comparatively high expression level ... | 2017 | 28689775 |