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molecular and antigenic characterization of bordetella bronchiseptica isolated from a wild southern sea otter (enhydra lutris nereis) with severe suppurative bronchopneumonia.bordetella bronchiseptica was isolated in pure culture from the lung, abdomen, and intestine of a wild free-ranging southern sea otter (enhydra lutris nereis) with severe, suppurative bronchopneumonia. immunohistochemistry, using antiserum raised to b. bronchiseptica, revealed strong positive staining of bacteria attached to bronchial ciliated epithelia as well as scattered positive staining in affected alveoli. western blot analysis demonstrated that virulence factors, filamentous hemagglutinin ...200314667021
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