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development of a protocol for the identification of tospoviruses and thrips species in individual thrips.a protocol for identifying tospovirus and thrips species in an individual thrips sample was successfully developed. first, an individual thrips was soaked in an rna stabilization solution to preserve protein and nucleic acids and ground in a carbonate buffer containing 0.2% sodium diethyldithiocarbamate. initially, the thrips extracts were screened for tospovirus infection by dot blot analysis using antibodies to nucleocapsid (n) proteins of tospoviruses. thrips extracts with positive results by ...201526141731
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