endosymbionts of ticks and their relationship to wolbachia spp. and tick-borne pathogens of humans and animals.the presence, internal distribution, and phylogenetic position of endosymbiotic bacteria from four species of specific-pathogen-free ticks were studied. these included the hard ticks ixodes scapularis (the black-legged tick), rhipicephalus sanguineus (the brown dog tick), and haemaphysalis longicornis and the african soft tick ornithodoros moubata. pcr assays for bacteria, using two sets of general primers for eubacterial 16s and 23s rrna genes (rdnas) and seven sets of specific primers for wolb ...19979327557
detection of francisella-like endosymbiont in hyalomma rufipes from ethiopia.the expanding family of francisellaceae includes the genus francisella, where several pathogen bacteria, e.g. the zoonotic f. tularensis, and different francisella-like agents belong to. francisella-like endosymbionts (fles) are widespread in hard and soft ticks and their pathogenicity is unknown. the examination of 296 ticks collected in ethiopia was performed for the detection of f. tularensis and fles using polymerase chain reaction (pcr) assays based on the amplification of 16s rrna, sdha an ...201425108781
rhipicephalus rossicus and not r. sanguineus is the dominant tick species of dogs in the wetlands of the danube delta, romania.changes in the distribution of tick species are among the major causes for the increase in prevalence of zoonotic diseases worldwide, with tick-borne diseases' prevalence showing an emerging pattern. one of these ticks, rhipicephalus rossicus, which is reported occasionally from humans, seems to be particularly interesting because of its demonstrated vectorial role for zoonotic pathogens like francisella tularensis, coxiella burnetii, or cchf and west nile viruses. here we report a case of domin ...201424893695
molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from china evaluated by nested investigation was performed to detect eight pathogens in ticks collected from grass tips or animals in the southern, central and northeast regions of china. dna samples extracted from ticks were collected from ten different locations in eight provinces of china and subjected to screening for tick-borne pathogens, including borrelia burgdorferi sensu lato, ehrlichia spp., rickettsia spp., babesia/theileria spp., ehrlichia ruminantium, coxiella burnetii, and francisella tularensis, using nested ...201626943995
tick-borne zoonotic bacteria in ticks collected from central spain.the prevalence of tick-borne and related bacteria infecting adult ticks in central spain was assessed by molecular methods. six areas were sampled monthly during a 2-year longitudinal study. a total of 1,038 questing and 442 feeding ticks, belonging to eight different species, were tested. the most abundant species were hyalomma lusitanicum (54% of captures), followed by dermacentor marginatus (23%) and rhipicephalus sanguineus (10%). four human pathogens, including seven rickettsia species, ana ...200919556569
rhipicephalus rossicus, a neglected tick at the margin of europe: a review of its distribution, ecology and medical importance.rhipicephalus rossicus (ixodida: ixodidae) is a three-host tick with a broad host spectrum that includes wild animals, pets, livestock and humans. despite its local abundance in certain areas, most of the available information on r. rossicus was published decades ago, mainly by former soviet authors. its distribution largely overlaps the eurasian steppe. however, its range may be more extensive than is currently known because this species may have been misidentified as rhipicephalus sanguineus, ...201525761411
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