minimising losses caused by zucchini yellow mosaic virus in vegetable cucurbit crops in tropical, sub-tropical and mediterranean environments through cultural methods and host resistance.between 2006 and 2009, 10 field experiments were done at kununurra, carnarvon or medina in western australia (wa) which have tropical, sub-tropical and mediterranean climates, respectively. these experiments investigated the effectiveness of cultural control measures in limiting zymv spread in pumpkin, and single-gene resistance in commercial cultivars of pumpkin, zucchini and cucumber. melon aphids (aphis gossypii) colonised field experiments at kununurra; migrant green peach aphids (myzus pers ...201121549770
the helper-component protease transmission factor of tobacco etch potyvirus binds specifically to an aphid ribosomal protein homologous to the laminin receptor precursor.potyviruses are plant pathogens transmitted by aphids in a non-persistent manner. during transmission, the virus-encoded factor helper-component protease (hcpro) is presumed to act as a molecular bridge, mediating the reversible retention of virions to uncharacterized binding sites in the vector mouthparts. whilst the predicted interaction between hcpro and the coat protein (cp) of virions has been confirmed experimentally, the characterization of putative hcpro-specific receptors in aphids has ...201020631085
intraspecific variation in zucchini yellow mosaic virus transmission by myzus persicae and the impact of aphid host plant.three isofemale lines of myzus persicae (sulzer), two lines collected from and reared on a brassicaceous host, and one line collected from and reared on a malvaceous host, were evaluated for their efficiency of transmitting zucchini yellow mosaic virus (family potyviridae, genus potyvirus, zymv). in the first experiment, the transmission efficiencies of two clones from brassicaceae (b1 and b2) were 52.0 and 60.8%, respectively, and these transmissions were not significantly different. in a secon ...200718232392
in vitro association between the helper component-proteinase of zucchini yellow mosaic virus and cuticle proteins of myzus persicae.potyviruses, as typical non-persistently transmitted viruses, are carried within the stylets of aphids. cuticle proteins (cups), which are a major component of the insect cuticle, were examined for in vitro binding to the potyviral helper component-proteinase (hc-pro). proteins in 8 m urea extracts from myzus persicae were separated by sds-page, electroblotted onto membranes and identified as cups by using specific antibodies to m. persicae cup. blotted m. persicae protein extracts were overlaid ...200717412993
comparative spatial spread overtime of zucchini yellow mosaic virus (zymv) and watermelon mosaic virus (wmv) in fields of transgenic squash expressing the coat protein genes of zymv and wmv, and in fields of nontransgenic squash.the spatial and temporal patterns of aphid-vectored spread of zucchini yellow mosaic virus (zymv) and watermelon mosaic virus (wmv) were monitored over two consecutive years in plantings of nontransgenic and transgenic squash zw-20h (commercial cv. freedom ii) and zw-20b, both expressing the coat protein genes of zymv and wmv. all test plants were surrounded by nontransgenic plants that were mechanically inoculated with zymv or wmv, and served as primary virus source. across all trials, none of ...200616838196
aphid transmission of a potyvirus depends on suitability of the helper component and the n terminus of the coat protein.the present study investigates the specificity of potyviruses for aphid species. two potyviruses differing in their host range were used: zucchini yellow mosaic virus (zymv) mainly infecting cucurbits and turnip mosaic virus (tumv) mainly infecting crucifers. two sets of aphids species were used as vectors, one polyphagous (myzus persicae and aphis gossypii) and the other from crucifers (brevicoryne brassicae and lipaphis erysimi). evidence is provided that the specificity between a vector and a ...200515503223
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