Publications

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identification and antigenic typing of rickettsia tsutsugamushi in naturally infected chiggers (acarina: trombiculidae) by direct immunofluorescence.rickettsia tsutsugamushi organisms were detected and typed antigenically by direct immunofluorescence in mites from laboratory-maintained infected colonies of leptotrombidium (leptotrombidium) fletcheri and l. (l.) arenicola. rickettsiae were identified most readily in unengorged larvae, but were also discernable in engorged larvae and all post-larval stages of the vectors.1978103449
the life history of a colony of leptotrombidium (leptotrombidium) fletcheri (womersley & heaslip) infected with rickettsia tsutsugamushi.the life cycle of 5 generations of leptotrombidium (l.) fletcheri infected with rickettsia tsutsugamushi and reared under ambient temperatures in malaysia was presented and compared with a colony reared at a constant 27 degrees c (neal and barnett, 1961). in general our colony had a longer generation time (average of 54 days from engorged larvae to adult compared with 37 days) and produced fewer eggs (average of 127.9 compared with 900.0) than the comparison colony. possible factors causing thes ...1977411178
distribution of rickettsia tsutsugamushi in organs of leptotrombidium (leptotrombidium) fletcheri (prostigmata: trombiculidae). 1975810585
identification of rickettsia tsutsugamushi in the life stages of leptotrombidium fletcheri with isolation and immunofluorescence techniques. 1975829477
development and evaluation of dot-immunobinding assay for detection of scrub typhus infection in leptotrombidium fletcheri (acari: trombiculidae).a dot-immunobinding assay (diba) was compared with a direct fluorescent antibody technique (dfat) for the detection of rickettsia tsutsugamushi infection in leptotrombidium fletcheri (womersley & heaslip). laboratory colonies of infected and noninfected chiggers were examined. the relative proportions of positive, negative, and indeterminate results were significantly different between diba and dfat for infected but not for noninfected chiggers. diba was more sensitive and had a better negative ...19921495069
doxycycline propylaxis for human scrub typhus.the use of doxycycline, as a prophylactic antibiotic against scrub typhus was investigated in a prospective, randomized, double-blind study. twenty volunteers were divided into two similar groups. beginning three days before exposure to leptotrombidium fletcheri chiggers infected with rickettsia tsutsugamushi, and continuing for six weeks after exposure, one group received weekly 200-mg oral doses of doxycycline and the other group received a placebo. nine of 10 doxycycline-treated subjects rema ...19826815282
detection and characterization of rickettsia tsutsugamushi (rickettsiales: rickettsiaceae) in infected leptotrombidium (leptotrombidium) fletcheri chiggers (acari: trombiculidae) with the polymerase chain reaction.we developed a method for detecting and characterizing the dna of rickettsia tsutsugamushi in chiggers (larval trombiculid mites) by polymerase chain reaction (pcr). three procedures for extracting dna from frozen chiggers were compared by evaluating the yield of pcr amplicand obtained with nine oligonucleotide primer pairs derived from the rickettsial 22 kd, 47 kd, groesl, 56 kd, and 110 kd antigen genes. although extracts and primer pairs differed in amplification efficiency, r. tsutsugamushi ...19947966172
laboratory tests of arthropod repellents against leptotrombidium deliense--noninfected and infected with rickettsia tsutsugamushi--and noninfected l. fletcheri (acari: trombiculidae).laboratory tests were conducted to compare the response of noninfected leptotrombidium deliense sambon and leptotrombidium fletcheri (womersley & heaslip) and l. deliense naturally infected with rickettsia tsutsugamushi, the etiologic agent of scrub typhus, to 8 chemical repellents and toxicants. low concentrations of permethrin, dimethylphthalate, diethyl methylbenzamide, benzyl benzoate, di-n-propyl 2,5-pyridine-dicarboxylate, 1-(3-cyclohexen-1-yl-carbonyl)-2-methylpiperidine (ai3-37220), 2-hy ...19968742527
occurrence of high ratio of males after introduction of minocycline in a colony of leptotrombidium fletcheri infected with orientia tsutsugamushi.in colonies of leptotrombidium fletcheri mites infected with orientia tsutsugamushi (ot), the agent of scrub typhus, males rarely appear. in the present study, the development of a high ratio of males was observed after introduction of minocycline (mc). a high dose of mc was injected subcutaneously into a mouse, and by feeding unfed larvae from an infected mite colony on this mouse, the ot in the mites were successfully killed. of a total of 130 unfed larvae attached to the mouse, 29 developed i ...19979062784
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