involvement of birds in the epidemiology of the lyme disease agent borrelia burgdorferi.borrelia burgdorferi, the causative agent of lyme disease, was isolated from the liver of a passerine bird, catharus fuscescens (veery), and from larval ixodes dammini (tick) feeding on pheucticus ludovicianus (rose-breasted grosbeak) and geothlypis trichas (common yellowthroat). in indirect immunofluorescence antibody tests, isolates reacted with polyclonal and monoclonal (h5332) antibodies. studies on the dna composition of the veery liver isolate and the strain cultured from an i. dammini lar ...19863943893
antibody response in lyme disease: evaluation of diagnostic tests.the antibody response to the ixodes dammini spirochete was determined in 41 serial serum samples from 12 patients with lyme disease. by enzyme-linked immunosorbent assay (elisa), 11 of the 12 patients had higher titers of specific igm antibody (greater than 1:200) during early disease than did 40 control subjects. specific igm antibody titers, which correlated with total amounts of igm antibody (p less than .001), sometimes remained elevated throughout the illness. during neuritis, nine of 10 pa ...19846373966
the antibody response in lyme disease.we determined the antibody response against the ixodes dammini spirochete in lyme disease patients by indirect immunofluorescence and an enzyme-linked immunosorbent assay (elisa). the specific igm response became maximal three to six weeks after disease onset, and then declined, although titers sometimes remained elevated during later disease. specific igm levels correlated directly with total serum igm. the specific igg response, often delayed initially, was nearly always present during neuriti ...19846393607
an ospa antigen-capture enzyme-linked immunosorbent assay for detecting north american isolates of borrelia burgdorferi in larval and nymphal ixodes antigen-capture enzyme-linked immunosorbent assay (elisa) was developed for detecting north american isolates of borrelia burgdorferi in larval, nymphal, and adult ticks. the assay uses an anti-ospa monoclonal antibody (h5332) for antigen capture and biotin-labelled polyclonal sera with streptavidinhorseradish peroxidase for signal generation. the assay recognized 15 of 15 north american b. burgdorferi isolates and did not cross-react with spirochete antigens of borrelia hermsii, borrelia tur ...19938432812
standardization of medium for culturing lyme disease standardize the procedure for isolating and culturing lyme disease spirochetes, we modified the composition of the medium generally used for this purpose (bsk-ii) and developed a system for its distribution. this medium contains no gelatin or agarose, and various components are used in proportions that differ from those in bsk-ii. each of the major proteinacious components was screened by substitution in samples of the complete product. the final medium was evaluated for the capacity to grow ...19938501226
ticks and borrelia: model systems for investigating pathogen-arthropod interactions.blood-feeding arthropods transmit numerous types of infectious agent and parasite that have a tremendous impact on human health and mortality throughout the world. these vector-borne pathogens display a wide array of evolutionary patterns that allow them to infect and to be successfully transmitted by ticks, mites, and hematophagous insects. the vector's method of feeding, type of development, and host preference are also critical factors for the transfer of zoonotic agents from wild animal rese ...19968805079
isolation, cultivation, and characterization of borrelia burgdorferi from rodents and ticks in the charleston area of south carolina.twenty-eight borrelia burgdorferi isolates from the charleston, s.c., area are described. this represents the first report and characterization of the lyme disease spirochete from that state. the isolates were obtained from december 1994 through december 1995 from the tick ixodes scapularis, collected from vegetation, and from the rodents peromyscus gossypinus (cotton mouse), neotoma floridana (eastern wood rat), and sigmodon hispidus (cotton rat). all isolates were screened immunologically by i ...200010618074
improving the specificity of 16s rdna-based polymerase chain reaction for detecting borrelia burgdorferi sensu lato-causative agents of human lyme disease.16s rdna sequences of borrelia burgdorferi sensu lato were aligned with the 16s rdna sequences of borrelia hermsii, borrelia turicatae, and borrelia lonestari in order to identify primers that might be used to more specifically identify agents of human lyme disease in ticks in human skin samples.200515752343
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