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detection of francisella tularensis and analysis of bacterial growth in ticks in japan.francisella tularensis is distributed in the northern hemisphere and it is the bacterial agent responsible for tularaemia, a zoonotic disease. we collected 4 527 samples of dna from ticks in japan, which were then analysed by real-time pcr and nested pcr. francisella dna was detected by real-time pcr in 2·15% (45/2 093) of ixodes ovatus, 0·66% (14/2 107) of i. persulcatus, 8·22% (6/73) of i. monospinosus and 0·72% (1/138) of haemaphysalis flava specimens. finally, francisella dna was detected by ...201627432517
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