| a new method for isolating trypanosoma brucei gambiense from sleeping sickness patients. | low infectivity to laboratory mammals and low virulence make trypanosoma brucei gambiense difficult to isolate and grow in amounts sufficient for biochemical characterization. we report the isolation of t.b. gambiense by feeding cryopreserved primary isolates to laboratory-reared glossina morsitans morsitans, followed by rapid cultivation in vitro of procyclic forms dissected from infected tsetse fly midguts. this technique allows the characterization of hitherto unsampled populations and avoids ... | 1989 | 2617625 |
| production of metacyclic forms by cyclical transmission of west african trypanosoma (t.) brucei isolates from man and animals. | fifteen west african trypanosoma (t.) brucei isolates from man and animals were cyclically transmitted. five stocks, belonging to the non-gambiense group, could easily be transmitted through glossina morsitans morsitans or glossina m. centralis infected on mice, whereas successful transmission of the 10 isolates, identified as trypanosoma brucei gambiense, was performed using g. palpalis gambiensis as vector. glossina p. gambiensis was infected with culture-derived procyclic trypanosomes by repe ... | 1988 | 2467539 |
| electron-microscopic localization of trypanosoma brucei gambiense transmitted by glossina morsitans centralis in microtus montanus. | in microtus montanus infected with t. b. gambiense, electron microscopic examination of lymph nodes, spleen, liver, heart, choroid plexus and brain demonstrated extravascular populations of trypanosomes distributed throughout interstitial spaces, accompanied by a moderate cellular infiltration of plasma cells. the trypanosomes exhibited numerous profiles; some were dividing, others were in different stages of lysis, or phagocytosed. penetration of trypanosomes into hepatocytes was observed. the ... | 1984 | 6152114 |
| inhibition of the dna amplification of trypanosomes present in tsetse flies midguts: implications for the identification of trypanosome species in wild tsetse flies. | the present study was carried out in order to investigate if there was really a failure of pcr in identifying parasitologically positive tsetse flies in the field. tsetse flies (glossina palpalis gambiensis and glossina morsitans morsitans) were therefore experimentally infected with two different species of trypanosoma (trypanosoma brucei gambiense or trypanosoma congolense). a total of 152 tsetse flies were dissected, and organs of each fly (midgut, proboscis or salivary glands) were examined. ... | 2004 | 15071836 |
| the isolation and genetic heterogeneity of trypanosoma brucei gambiense from north-west uganda. | fifty-two samples of blood were taken from sleeping sickness patients in north-west uganda. all samples failed to infect immunosuppressed mice. ten cryopreserved blood samples were fed to laboratory bred glossina morsitans morsitans; eight flies developed midgut infections from which procyclic cultures were established in vitro. isoenzyme electrophoretic analysis of 9 enzymes revealed that 7 of the 8 trypanosome isolates had a combination of enzyme patterns already described for trypanosoma bruc ... | 1993 | 8103626 |
| comparative genomics of glossina palpalis gambiensis and g. morsitans morsitans to reveal gene orthologs involved in infection by trypanosoma brucei gambiense. | blood-feeding glossina palpalis gambiense (gpg) fly transmits the single-celled eukaryotic parasite trypanosoma brucei gambiense (tbg), the second glossina fly african trypanosome pair being glossina morsitans/t.brucei rhodesiense. whatever the t. brucei subspecies, whereas the onset of their developmental program in the zoo-anthropophilic blood feeding flies does unfold in the fly midgut, its completion is taking place in the fly salivary gland where does emerge a low size metacyclic trypomasti ... | 2017 | 28421044 |
| midgut expression of immune-related genes in glossina palpalis gambiensis challenged with trypanosoma brucei gambiense. | tsetse flies from the subspecies glossina morsitans morsitans and glossina palpalis gambiensis, respectively, transmit trypanosoma brucei rhodesiense and trypanosoma brucei gambiense. the former causes the acute form of sleeping sickness, and the latter provokes the chronic form. although several articles have reported g. m. morsitans gene expression following trypanosome infection, no comparable investigation has been performed for g. p. gambiensis. this report presents results on the different ... | 2014 | 25426112 |