in vitro identification of two adherence factors required for in vivo virulence of pseudomonas enriching a random transposon insertion bank of pseudomonas fluorescens for mutants affected in their adherence to the human extracellular matrix protein fibronectin, we isolated 23 adherence minus mutants. mutants showed a defect in their ability to develop a biofilm on an abiotic surface and were impaired for virulence when tested in an in vivo virulence model in the fruit fly, drosophila melanogaster. molecular characterisation of these mutants showed that the transposon insertions localis ...200314623013
lethality and developmental delay in drosophila melanogaster larvae after ingestion of selected pseudomonas fluorescens strains.the fruit fly, drosophila melanogaster, is a well-established model organism for probing the molecular and cellular basis of physiological and immune system responses of adults or late stage larvae to bacterial challenge. however, very little is known about the consequences of bacterial infections that occur in earlier stages of development. we have infected mid-second instar larvae with strains of pseudomonas fluorescens to determine how infection alters the ability of larvae to survive and com ...201020856932
characterization of toxin complex gene clusters and insect toxicity of bacteria representing four subgroups of pseudomonas fluorescens.ten strains representing four lineages of the pseudomonas fluorescens group (p. chlororaphis, p. corrugata, p. koreensis, and p. fluorescens subgroups) were evaluated for toxicity to the tobacco hornworm manduca sexta and the common fruit fly drosophila melanogaster. the three strains within the p. chlororaphis subgroup exhibited both oral and injectable toxicity to the lepidopteran m. sexta. all three strains have the gene cluster encoding the fitd insect toxin and a ╬┤fitd mutant of p. protegen ...201627580176
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