Publications

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drosophila melanogaster as a model for elucidating the pathogenicity of francisella tularensis.drosophila melanogaster is a widely used model organism for research on innate immunity and serves as an experimental model for infectious diseases. the aetiological agent of the zoonotic disease tularaemia, francisella tularensis, can be transmitted by ticks and mosquitoes and drosophila might be a useful, genetically amenable model host to elucidate the interactions between the bacterium and its arthropod vectors. we found that the live vaccine strain of f. tularensis was phagocytosed by droso ...200818248629
intracellular fate of francisella tularensis within arthropod-derived cells.since transmission of francisella tularensis into the mammalian host occurs via arthropod vectors such as ticks, mosquitoes, horseflies and deerflies, recent studies have established drosophila melanogaster as an arthropod vector model system. nothing is known about the intracellular fate of f. tularensis within arthropod-derived cells, and the role of this host-parasite adaptation in the evolution of this pathogen to infect mammals. in this report, we explored intracellular trafficking of f. tu ...200919220402
directed screen of francisella novicida virulence determinants using drosophila melanogaster.francisella tularensis is a highly virulent, facultative intracellular human pathogen whose virulence mechanisms are not well understood. occasional outbreaks of tularemia and the potential use of f. tularensis as a bioterrorist agent warrant better knowledge about the pathogenicity of this bacterium. thus far, genome-wide in vivo screens for virulence factors have been performed in mice, all however restricted by the necessity to apply competition-based, negative-selection assays. we wanted to ...201020479082
molecular bases of proliferation of francisella tularensis in arthropod vectors.arthropod vectors are important vehicles for transmission of francisella tularensis between mammals, but very little is known about the f. tularensis-arthropod vector interaction. drosophila melanogaster has been recently developed as an arthropod vector model for f. tularensis. we have shown that intracellular trafficking of f. tularensis within human monocytes-derived macrophages and d. melanogaster-derived s2 cells is very similar. within both evolutionarily distant host cells, the francisell ...201020482589
host factors required for modulation of phagosome biogenesis and proliferation of francisella tularensis within the cytosol.francisella tularensis is a highly infectious facultative intracellular bacterium that can be transmitted between mammals by arthropod vectors. similar to many other intracellular bacteria that replicate within the cytosol, such as listeria, shigella, burkholderia, and rickettsia, the virulence of f. tularensis depends on its ability to modulate biogenesis of its phagosome and to escape into the host cell cytosol where it proliferates. recent studies have identified the f. tularensis genes requi ...201020552012
francisella-arthropod vector interaction and its role in patho-adaptation to infect mammals.francisella tularensis is a gram-negative, intracellular, zoonotic bacterium, and is the causative agent of tularemia with a broad host range. arthropods such as ticks, mosquitoes, and flies maintain f. tularensis in nature by transmitting the bacteria among small mammals. while the tick is largely believed to be a biological vector of f. tularensis, transmission by mosquitoes and flies is largely believed to be mechanical on the mouthpart through interrupted feedings. however, the mechanism of ...201121687425
francisella is sensitive to insect antimicrobial peptides.francisella tularensis causes the zoonotic disease tularemia. arthropod vectors are important transmission routes for the disease, although it is not known how francisella survives the efficient arthropod immune response. here, we used drosophila melanogaster as a model host for francisella infections and investigated whether the bacteria are resistant to insect humoral immune responses, in particular to the antimicrobial peptides (amps) secreted into the insect hemolymph. moreover, we asked to ...201323037919
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