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large scale production of bacillus thuringiensis ps149b1 insecticidal proteins cry34ab1 and cry35ab1 from pseudomonas fluorescens.the 14kda (cry34ab1) and 44kda (cry35ab1) binary insecticidal proteins are produced naturally by bacillus thuringiensis ps149b1 as parasporal inclusion bodies. here, we show production of these two insecticidal proteins in recombinant pseudomonas fluorescens and their subsequent purification to near homogeneity to provide large quantities of protein for safety-assessment studies associated with the registration of transgenic corn plants. the gene sequence specific for each protein was expressed ...200717337206
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