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isolation of chuzan virus, a new member of the palyam subgroup of the genus orbivirus, from cattle and culicoides oxystoma in japan.five virus strains with identical antigenic properties were isolated from 3 rbc suspensions obtained from 2 healthy sentinel calves and from 2 pools of culicoides oxystoma in cultures of a hamster lung cell line (hmlu-1). the virus was tentatively named chuzan virus. the chuzan virus was classified as a new member of the palyam subgroup of the genus orbivirus on the basis of its physicochemical, morphologic, and antigenic properties.19883239836
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