| [isolation and identification of the akabane virus from mosquitoes in yunnan province, china]. | to evaluate the prevalence of mosquito-borne viruses in manshi and ruili (yunnan province, china), we collected 2 149 mosquitoes (17 species) in august 2010. virus isolation was undertaken by the cul- ture of baby hamster kidney cells (bhk-21 cells). two virus-like isolates were obtained: dhl10m117 was isolated from collected in mangshi; dhl10m110 was obtained from anopheles vagus collected in rui- li. both isolates caused cytopathic effects,illness and death in suckling mice inoculated with the ... | 2015 | 25997331 |
| [isolation and identification of arbovirus in hainan province, 2017-2018]. | objective: to understand the types and distribution of arboviruses in hainan province. methods: blood-sucking insects were collected in hainan province from 2017 to 2018. after laboratory treatment, bhk-21 cells and c6/36 cells were inoculated with grinding supernatant of all blood-sucking insects to isolate all of involving virus. arbovirus genes in blood-sucking insects were detected in parallel by rt-pcr method. results: a total of 15 062 mosquitoes were classified into four genera (culex, ar ... | 2020 | 32164136 |
| [mosquitoes, midges and related arboviruses in southeast sichuan province]. | objective: to investigate the distribution patterns of mosquitoes, midges and related arboviruses in sichuan province. methods: blood-sucking insects were collected from houses and pens, using the ultraviolet lights. mosquito samples were classified according to morphologic characteristics and then stored at liquid nitrogen. all samples were incubated with bhk-21 and c6/36 cells for virus isolation and then detected for their viral genes. sequences of the virus were identified and analyzed by mo ... | 2018 | 30453441 |