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development of conventional and real-time reverse transcription polymerase chain reaction assays to detect tembusu virus in culex tarsalis mosquitoes.tembusu virus (tmuv) is an important emerging arthropod-borne virus that may cause encephalitis in humans and has been isolated in regions of southeast asia, including malaysia, thailand, and china. currently, detection and identification of tmuv are limited to research laboratories, because quantitative rapid diagnostic assays for the virus do not exist. we describe the development of sensitive and specific conventional and real-time quantitative reverse transcription polymerase chain reaction ...201425114013
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